• Characterization of Insect Immune Systems from Genomic Data.- Analyzing Immunity in Non-model Insects using de novo Transcriptomics.- Preparation of Insect Protein Samples for Label-free Proteomic Quantification by LC-mass Spectrometry.- Quantification of Blood Cells in Drosophila melanogaster and other Insects.- Methods to Quantify in vivo Phagocytic Uptake and Opsonization of Live or Killed microbes in Drosophila melanogaster.- Analysis of Cellular Immune Responses in Lepidopteran Larvae.- Detection of Enzyme Distribution, Expression, Activation, and Activity of Insect Prophenoloxidase.- Basic Methods to Evaluate Humoral Immunity Processes in Lepidoptera larvae.- Methods for the in vitro Examination of the Antibacterial and Cytotoxic Activities of Antimicrobial Peptides.- Characterization of Antimicrobial Peptide - Membrane Interaction Using All-atom Molecular Dynamic Simulation.- Manipulating the Mosquito Microbiota to Study its Function.- Discovery and Analysis of RNA Viruses in Insects.- A Workflow for Infection of Drosophila with Entomopathogenic Nematodes to Monitor Immune Gene Transcriptional Activity.- Oral Infection in a Germ-Free Bombyx mori Model.- Silkworm Infection Model for Evaluating Pathogen Virulence.

“With its broad and lucid treatment of insect immunity, this volume paves the way for new recruits and encourages current researchers to expand and refine their approaches.” (James B. Nardi, The Quarterly Review of Biology, Vol. 95 (4), December, 2020)