Immobilization of Enzymes and Cells

Methods and Protocols

Häftad, Engelska, 2021

AvJose M. Guisan,Juan M. Bolivar,Fernando López-Gallego,Javier Rocha-Martín,Fernando Lopez-Gallego,Javier Rocha-Martin

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This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells.

Produktinformation

Utgivningsdatum2021-01-15
Mått178 x 254 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor495
Upplaga4
FörlagSpringer-Verlag New York Inc.
ISBN9781071602171

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Overview of Immobilized Enzymes Applications in Pharmaceutical, Chemical, and Food Industry.- Parameters for the Evaluation of Immobilized Enzymes under Process Conditions.- One-Point Covalent Immobilization of Enzymes on Glyoxyl-Agarose with Minimal Physico-Chemical Modification: Immobilized “Native Enzymes”.- Multi-Point Covalent Immobilization of Enzymes on Glyoxyl-Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes.- Multi-Potent Covalent Immobilization of Enzymes on Supports Activated with Epoxy Groups: Stabilization of Industrial Enzymes.- Immobilization of Enzymes on Supports Activated with Glutaraldehyde: A Very Simple Immobilization Protocol.- Very Strong but Reversible Immobilization of Enzymes on Supports Coated with Ionic Polymers.- Immobilization of Lipases by Adsorption on Hydrophobic Supports: Modulation of Enzyme Properties in Biotransformations in Anhydrous Media.- Immobilization of Enzymes on Hetero-Functional Supports: Physical Adsorption Plus Additional Covalent Immobilization.- Stabilization of Multimetic Enzymes via Immobilization and Further Cross-Linking with Aldehyde-Dextran.- Immobilization of Enzymes on Magnetic Beads through Affinity Interactions.- Immobilization of Proteins on Gold Surfaces.- Immobilization of Enzymes in Protein Films.- Encapsulation of Enzymes in Porous Capsules via particle Templating.- Enzyme Immobilization in Wall-Coated Flow Microreactors.- In Situ Immobilization of Enzymes in Biomimetic Silica.- Environmentally-Friendly Enzyme Immobilization onto MOF Materials.- Co-Immobilization and Co-Localization of Multi-Enzymes Systems on Porous Materials.- Single-Particle Kinetics of Immobilized Enzymes by Harnessing the Autofluorescence of Co-Immobilized Cofactors.- Intraparticle pH Sensing within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles.- Manufacturing of Protein-Based Biomaterials Coupling Cell-Free Protein Synthesis with Protein Immobilization.- Immobilization of Enzymes as Cross-Linked Enzyme Aggregates: General Strategy to Obtain Robust Biocatalysts.- NMR OF Immobilized Enzymes.- Whole Cell Entrapment Techniques.- Immobilization of INS1E Insulin-Producing Cells within Injectable Alginate Hydrogels.- Immobilization of Cells on Polyurethane Foam.- Covalent Immobilization of Microbial Cells on Microchannel Surfaces.- Immobilization of Cells by Magnetic Nanoparticles.- Cultivation of Productive Biofilms in Flow-Reactors and their Characterization by CLSM.- Immobilization of Microalgae.- Therapeutic Applications of Encapsulated Cells.