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The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons.
Quantification of Autophagosome Size and Formation Rate by Electron and Fluorescence Microscopy in Baker’s Yeast.- Ultrastructure of the Macroautophagy Pathway in Mammalian Cells.- Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons.- Monitoring Autophagic Activity In Vitro and In Vivo using the GFP-LC3-RFP-LC3∆G Probe.- Measuring Autophagic Flux in Neurons by Optical Pulse Labeling.- Measuring Autophagosome Flux.- Measurement of Neuronal Tau Clearance In Vivo.- Methods for Studying Axonal Autophagosome Dynamics in Adult Dorsal Root Ganglion Neurons.- Imaging and Quantifying Neuronal Autophagy to Determine the Autophagy Contribution to Neuronal and Dendritic Morphogenesis.- Correlative Light and Electron Microscopy (CLEM): Bringing Together the Best of Both Worlds to Study Neuronal Autophagy.
