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< Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function.
Introduction.- Preparing the hydrocarbon / crude oil.- High pressure cultivation of hydrocarbonoclastic aerobic bacteria.- In vitro high pressure incubation and activity measurement of deep-sea, methanogenic archaea.- Bioreactor design to emulate deep-sea hydrocarbon releases including formation of gas hydrates.- Mudflat benthic spill simulations.- Protocols for subtidal and deep sea benthic oil spill simulations.- Simulation of anoxic/oxic oscillations in crude oil-degrading bioreactors.- Protocols for microcosms for growing biofilms on hydrophobic substrates – a polyphasic approach to study biodiversity, metabolic activity and biofilm architecture.- Application of micro- and mesocosms experiments to pollutant effects in biofilms.- Mini sediment columns and two-dimensional sediment flow-through microcosms - versatile model systems for studying biodegradation of organic contaminants in groundwater ecosystems.