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This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds.
Introduction.- Protocols for the isolation and analysis of lipopeptides and bioemulsifiers.- Protocols for the detection and chemical characterisation of microbial glycolipids.- Isolation of glycoprotein bioemulsifiers produced by marine bacteria.- Protocols for lipid extraction from wet algal biomass.- Isolation and characterization of lipid droplets from yeast.- Protocols for Isolation and Analysis of Polyhydroxyalkanoates.- Purification of lipid rafts from bacterial membranes.- Isolation and analysis of membrane phospholipids as biomarkers.- Protocols for purifying and characterizing integral membrane AlkB enzymes.- Protocols for structural and functional analysis of particulate methane monooxygenase from Methylocystis species strain Rockwell (ATCC 49242).- Phenotype-based Identification of key enzymes for polycyclic aromatic hydrocarbon (PAH) metabolism from Mycobacteria using transposon mutagenesis and a PAH spray plate.- Microcalorimetry as a general technique to characterize ligand binding.