Introduction.- Designing bacteria to produce esters.- Protocols for the production and analysis of isoprenoids in bacteria and yeast.- Synthetic biology of hydrophobic polymer production.- Methods for recombinant rhamnolipid production.- Purification of peptide antimicrobials and thioether stabilized molecules produced in vivo by lantibiotic modification machineries.- Engineering transcription factor based biosensors for the detection of intracellular products.- Oxyfunctionalization of linear alkanes with a biosynthetic, self-sufficient, selective and soluble hydroxylase.