Human Embryonic Stem Cells

Stephen Sullivan: Trinity College, Dublin, Ireland  Kevin Eggan: Harvard University, Cambridge, MA, USAChad A. Cowan: Harvard University, Cambridge, MA, USA

• Foreword. Davor Solter Preface.Section 1: Obtaining and culturing human embryonic stem cells.1 Organization and good aseptic technique in the human embryonic stem cell laboratory.Minal J Patel, Emma L Stephenson, and Stephen L Minger2 Sourcing human embryonic stem cell lines.Glyn Stacey3 Human embryo culture for human embryonic stem cell derivation.R. Douglas Powers and Jeannine Witmyer4 Derivation of human embryonic stem cell lines.Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan and Chad A. Cowan5 Standard culture of human embryonic stem cells.Jeremy M. Crook, Rachel Horne, and Alan Colman6 Culture of human embryonic stem cells in chemically defined conditions.Julie Hsu Clark and Sheng DingSection 2: Characterization of undifferentiated embryonic stem cells.7 Phenotypic analysis of human embryonic stem cells.Jonathan S. Draper,  Cheryle A. Séguin and Peter W. Andrews8 Genetic and epigenetic analysis of human embryonic stem cells.Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova Section 3: Manipulation of human embryonic stem cells.9 In vivo differentiation of human embryonic stem cells.Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou10 In vitro differentiation of human embryonic stem cells.A. Henry Sathananthan and Alan Trounson11 Differentiation of human embryonic stem cells into extraembryonic cell types.Cheryle A. Séguin and Jonathan S. Draper12 Directed differentiation of human embryonic stem cells into:(a) Early endoderm cells.Kenji Osafune, Alice E. Chen and Douglas A. Melton(b) Hepatic cells.Neta Lavon and Nissim Benvenisty(c) Pancreatic cells.Hiram Chipperfield13 Directed differentiation of human embryonic stem cells into:(a) Cardiomyocytes.Christine Mummery, Robert Passier and Chris Denning(b) Endothelial cells.Carrie Soukup, Shulamit Levenberg and Ondine Cleaver(c) Osteogenic cells.Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini and Robert Langer(d) Hematopoietic in vivo repopulating cells.Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q. Daley(e) Lymphocytes.Petter S. Woll and Dan S. Kaufman(f) Myeloid cells.Chantal Cerdan and Mickie Bhatia14 Directed differentiation of human embryonic stem cells into:(a) Forebrain neurons.Emily A. Davis and Lawrence S.B. Goldstein(b) Dopaminergic neuron.Jan Pruszak and Ole Isacson(c) Spinal motor neurons.Bao-Yang Hu and Su-Chun Zhang15  (a) Gene targeting: knock out and knock in by homologous recombination.Thomas P. Zwaka(b) RNA interference in human embryonic stem cells.M. William Lensch, Asmin Tulpule and Holm Zaehres(c) Generation of gene reporters using bacterial artificial chromosome recombineering. Andrew J. Washkowitz and David A. ShaywitzAfterword. Azim SuraniIndex.

"A highly readable and useful book … .[It] will help many to navigate the uncharted waters of human embryonic stem cell biology."  (The British Society for Cell Biology Newsletter, Autumn, 2008) "[It] is a valuable resource for seasoned and novice researchers; it would make an excellent addition to the reference collection of any medical library or research laboratory." (American Medical Association, September 2008)"...the imaginative solutions in this book can inspire us to get past our most frustrating limitations." (Cell Stem Cell, September 2007)"...the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human ES cells..."  (Regenerative Medicine, 2007)"In this fast-moving field, this [handbook] will help drive advances of more and more researchers."  (Differentiation, 2007)"A highly readable and useful book ... [This book] will help many to navigate the uncharted waters of human embryonic stem cell biology." (British Society for Cell Biology, Autumn, 2008)