Del 550 - Methods in Molecular Biology

Human Embryogenesis

Methods and Protocols

Häftad, Engelska, 2014

Av Julie Lafond, Cathy Vaillancourt, Julie LaFond

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Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.

Produktinformation

Utgivningsdatum2014-10-30
Mått193 x 260 x 18 mm
Vikt679 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor312
Upplaga2009
FörlagHumana Press Inc.
ISBN9781627038324

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

Introduction: What is Human Embryogenesis?.- Human Embryogenesis: Overview.- Studying Embryo Implantation: From Trophoblasts to Embryo.- Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation.- Coculture of Decidua and Trophoblast to Study Proliferation and Invasion.- Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells’ Calcium Uptake.- Endocrinology and Cell Signaling in Human Villous Trophoblast.- Gestation-Induced Uterine Vascular Remodeling.- Steriodogenesis and Reproductive System.- Placental and Fetal Steroidogenesis.- Current Methods in Investigating the Development of the Female Reproductive System.- A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins.- A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis.- From Embryo to Fetal Development.- Genetic Dissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow Cytometry.- Immune System: Maturation of Myeloid Cells.- Functional Development of Human Fetal Gastrointestinal Tract.- Renal and Cardiac Na+–K+-ATPase and Aconitase in a Rat Model of Fetal Programming.- Embryotoxicity, in Vitro Fertilization, Cloning, and Ethics.- Assessment of Embryotoxicity Using Mouse Embryo Culture.- Fertilization In Vitro.- Animal Cloning by Somatic Cell Nuclear Transfer.- The Human Embryo: Ethical and Legal Aspects.

From the reviews:“This book in the Methods in Molecular Biology Series covers human embryogenesis with sections on gametes, fertilization, embryo development, implantation, and organ system development. … Bench researchers in a variety of disciplines would find the research protocols useful. … This unique book for researchers covers an array of topics that are generally not found together in one volume.” (Linda R. Nelson, Doody’s Review Service, March, 2010)“Human Embryogenesis offers useful and detailed descriptions of a variety of tissue preparation and analytic techniques. … may be helpful for investigators to determine whether some of the materials listed in the detailed protocols can be legally obtained in their jurisdiction. … This book would be useful for investigators who are moving into certain new areas of embryogenesis research, as well as post doctoral and graduate students with experimental interests closely aligned to the procedures that are included.” (Armand Lione, Birth Defects Research (Part A): Clinical and Molecular Teratology, Vol. 88, June, 2010)