Associate Prof. Christian Schwerk:Associate Prof. Dr. Christian Schwerk is a molecular and cellular biologist heading the infectious diseases research laboratory at the Department of Pediatrics, Pediatric Infectious Diseases, of the Medical Faculty Mannheim, Heidelberg University. He obtained his doctorate from the Institute of Cellular Biology (Cancer Research) at the University Hospital Essen, Germany and has worked in several international research institutions. Dr. Schwerk has published more than 70 peer reviewed publications and serves on the editorial boards of international journals.Prof. Horst SchrotenProfessor Dr. Horst Schroten is Director of the University Children's Hospital, Medical Faculty Mannheim, Heidelberg University. His scientific focus is on research into the pathogenesis of infectious diseases, in particular bacterial and viral meningitis. After holding a professorship for pediatric infectiology at the University of Düsseldorf, he was appointed to the Chair of General Paediatrics at the Mannheim Medical Faculty Heidelberg University on March 1, 2008. From 2001-2005 he was the President of the German Society for Pediatric Infectious Diseases and has published more than 200 peer-reviewed scientific publications.Prof. Hiroshi IshikawaProfessor Hiroshi Ishikawa graduated from The Jikei University School of Medicine in 1968, received Medical doctor Science 1972. In 1974, associate professor at Tohoku University School of Medicine. In 1985, appointed chairman of the Department of Histology and Embryology at The Jikei University School of Medicine. From 2003 to 2015 president of the Japanese Society of Human Cell. Since 2003 he has been on the Human Cell editorial board. He has deposited 84 cell lines in the Riken Cell Bank and has published over 490 papers. In 1984, he received the Research Encouragement Award from the Japan Endocrine Society, and since then he has received numerous academic awards. Currently, he has established a clinical regenerative medicine laboratory at the Department of Neurosurgery, University of Tsukuba. He conducts research on regenerative medicine based on embryology, especially on the treatment of cerebral infarction by transplanting differentiation-induced nervous system cells derived from human dental pulp.

• Part 1 BBB & BCSFB introductions.- 1 The History of the Blood-CNS-barriers.- Part 2 BCSFB/HIBCPP.-  2 An overview of human choroid plexus structure and transport functions.- 3 Detailed protocol for establishing HIBCPP cell line derived from human choroid plexus papilloma: Special reference to the neuroprotective effects of transthyretin produced by HIBCPP cells.- 4 Bitter taste receptors as gatekeepers of brain barriers.- 5 Changes in the Blood Choroid Plexus Barrier in Response to Transient Receptor Potential, Vanilloid 4 Channel Activation.- 6 The blood-cerebrospinal fluid barrier and brain manganese accumulation.- 7 "Autoimmune interactions of antibacterial antibodies with brain proteins on choroid plexus cells: is there a role for neurodevelopmental disorders?".- 8 Multiple Sclerosis and the blood‒cerebrospinal fluid barrier.- 9 Viral infections of the CNS – is it all about breaching barriers?.- 10 The Choroid Plexus: A Barrier to the Brain and a Target for Central Nervous System-Invading Bacteria.- 11 Brain Infection in African Sleeping Sickness.- 12 The Blood-CSF Barrier in Oncological Research.- Part 3 BBB.- 13 In vitro models of the blood-brain barrier to study multiple sclerosis pathogenesis.- 14 Construction and Functional Evaluation of 2D and 3D Human Blood-Brain Barrier Models.

Associate Prof. Christian Schwerk:Associate Prof. Dr. Christian Schwerk is a molecular and cellular biologist heading the infectious diseases research laboratory at the Department of Pediatrics, Pediatric Infectious Diseases, of the Medical Faculty Mannheim, Heidelberg University. He obtained his doctorate from the Institute of Cellular Biology (Cancer Research) at the University Hospital Essen, Germany and has worked in several international research institutions. Dr. Schwerk has published more than 70 peer reviewed publications and serves on the editorial boards of international journals.Prof. Horst SchrotenProfessor Dr. Horst Schroten is Director of the University Children's Hospital, Medical Faculty Mannheim, Heidelberg University. His scientific focus is on research into the pathogenesis of infectious diseases, in particular bacterial and viral meningitis. After holding a professorship for pediatric infectiology at the University of Düsseldorf, he was appointed to the Chair of General Paediatrics at the Mannheim Medical Faculty Heidelberg University on March 1, 2008. From 2001-2005 he was the President of the German Society for Pediatric Infectious Diseases and has published more than 200 peer-reviewed scientific publications.Prof. Hiroshi IshikawaProfessor Hiroshi Ishikawa graduated from The Jikei University School of Medicine in 1968, received Medical doctor Science 1972. In 1974, associate professor at Tohoku University School of Medicine. In 1985, appointed chairman of the Department of Histology and Embryology at The Jikei University School of Medicine. From 2003 to 2015 president of the Japanese Society of Human Cell. Since 2003 he has been on the Human Cell editorial board. He has deposited 84 cell lines in the Riken Cell Bank and has published over 490 papers. In 1984, he received the Research Encouragement Award from the Japan Endocrine Society, and since then he has received numerous academic awards. Currently, he has established a clinical regenerative medicine laboratory at the Department of Neurosurgery, University of Tsukuba. He conducts research on regenerative medicine based on embryology, especially on the treatment of cerebral infarction by transplanting differentiation-induced nervous system cells derived from human dental pulp.

• Part 1 BBB & BCSFB introductions.- 1 The History of the Blood-CNS-barriers.- Part 2 BCSFB/HIBCPP.-  2 An overview of human choroid plexus structure and transport functions.- 3 Detailed protocol for establishing HIBCPP cell line derived from human choroid plexus papilloma: Special reference to the neuroprotective effects of transthyretin produced by HIBCPP cells.- 4 Bitter taste receptors as gatekeepers of brain barriers.- 5 Changes in the Blood Choroid Plexus Barrier in Response to Transient Receptor Potential, Vanilloid 4 Channel Activation.- 6 The blood-cerebrospinal fluid barrier and brain manganese accumulation.- 7 "Autoimmune interactions of antibacterial antibodies with brain proteins on choroid plexus cells: is there a role for neurodevelopmental disorders?".- 8 Multiple Sclerosis and the blood‒cerebrospinal fluid barrier.- 9 Viral infections of the CNS – is it all about breaching barriers?.- 10 The Choroid Plexus: A Barrier to the Brain and a Target for Central Nervous System-Invading Bacteria.- 11 Brain Infection in African Sleeping Sickness.- 12 The Blood-CSF Barrier in Oncological Research.- Part 3 BBB.- 13 In vitro models of the blood-brain barrier to study multiple sclerosis pathogenesis.- 14 Construction and Functional Evaluation of 2D and 3D Human Blood-Brain Barrier Models.

Associate Prof. Christian Schwerk:Associate Prof. Dr. Christian Schwerk is a molecular and cellular biologist heading the infectious diseases research laboratory at the Department of Pediatrics, Pediatric Infectious Diseases, of the Medical Faculty Mannheim, Heidelberg University. He obtained his doctorate from the Institute of Cellular Biology (Cancer Research) at the University Hospital Essen, Germany and has worked in several international research institutions. Dr. Schwerk has published more than 70 peer reviewed publications and serves on the editorial boards of international journals.Prof. Horst SchrotenProfessor Dr. Horst Schroten is Director of the University Children's Hospital, Medical Faculty Mannheim, Heidelberg University. His scientific focus is on research into the pathogenesis of infectious diseases, in particular bacterial and viral meningitis. After holding a professorship for pediatric infectiology at the University of Düsseldorf, he was appointed to the Chair of General Paediatrics at the Mannheim Medical Faculty Heidelberg University on March 1, 2008. From 2001-2005 he was the President of the German Society for Pediatric Infectious Diseases and has published more than 200 peer-reviewed scientific publications.Prof. Hiroshi IshikawaProfessor Hiroshi Ishikawa graduated from The Jikei University School of Medicine in 1968, received Medical doctor Science 1972. In 1974, associate professor at Tohoku University School of Medicine. In 1985, appointed chairman of the Department of Histology and Embryology at The Jikei University School of Medicine. From 2003 to 2015 president of the Japanese Society of Human Cell. Since 2003 he has been on the Human Cell editorial board. He has deposited 84 cell lines in the Riken Cell Bank and has published over 490 papers. In 1984, he received the Research Encouragement Award from the Japan Endocrine Society, and since then he has received numerous academic awards. Currently, he has established a clinical regenerative medicine laboratory at the Department of Neurosurgery, University of Tsukuba. He conducts research on regenerative medicine based on embryology, especially on the treatment of cerebral infarction by transplanting differentiation-induced nervous system cells derived from human dental pulp.

• Part 1 BBB & BCSFB introductions.- 1 The History of the Blood-CNS-barriers.- Part 2 BCSFB/HIBCPP.-  2 An overview of human choroid plexus structure and transport functions.- 3 Detailed protocol for establishing HIBCPP cell line derived from human choroid plexus papilloma: Special reference to the neuroprotective effects of transthyretin produced by HIBCPP cells.- 4 Bitter taste receptors as gatekeepers of brain barriers.- 5 Changes in the Blood Choroid Plexus Barrier in Response to Transient Receptor Potential, Vanilloid 4 Channel Activation.- 6 The blood-cerebrospinal fluid barrier and brain manganese accumulation.- 7 "Autoimmune interactions of antibacterial antibodies with brain proteins on choroid plexus cells: is there a role for neurodevelopmental disorders?".- 8 Multiple Sclerosis and the blood‒cerebrospinal fluid barrier.- 9 Viral infections of the CNS – is it all about breaching barriers?.- 10 The Choroid Plexus: A Barrier to the Brain and a Target for Central Nervous System-Invading Bacteria.- 11 Brain Infection in African Sleeping Sickness.- 12 The Blood-CSF Barrier in Oncological Research.- Part 3 BBB.- 13 In vitro models of the blood-brain barrier to study multiple sclerosis pathogenesis.- 14 Construction and Functional Evaluation of 2D and 3D Human Blood-Brain Barrier Models.