Histidine Phosphorylation

Methods and Protocols

Häftad, Engelska, 2020

1 569 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.

This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine.

Produktinformation

Utgivningsdatum2020-11-10
Mått178 x 254 x 15 mm
Vikt511 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor256
FörlagHumana Press Inc.
ISBN9781493998869

Tillhör följande kategorier

Biokemi inom Naturvetenskap och teknik

Protein Dynamics in Phosphoryl-transfer Signaling Mediated by Two-component Systems.- A High-throughput Strategy for Recombinant Protein Expression and Solubility Screening in Escherichia coli – A Case of Sensor Histidine Kinase.- SDS-PAGE and Dot Blot Autoradiography: Tools for Quantifying Histidine Kinase Autophosphorylation.- A Quantitative Method for the Measurement of Protein Histidine Phosphorylation.- Analysis of 1- and 3-phosphohistidine (pHis) Protein Modification using Model Enzymes Expressed in Bacteria.- Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry.- Protein Phosphohistidine Phosphatases of the HP Superfamily.- In Vitro Assays for Measuring Protein Histidine Phosphatase Activity.- Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases.- Manipulation of Bacterial Signalling using Engineered Histidine Kinases.- Development of a Light-Dependent Protein Histidine Kinase.- Empirical Evidence of Cellular Histidine Phosphorylation by Immunoblotting using pHis mAbs.- Immunohistochemistry (IHC): Chromogenic Detection of 3-Phosphohistidine Proteins in Formaldehyde-Fixed, Frozen Mouse Liver Tissue Sections.- Subcellular Localisation of Histidine Phosphorylated Proteins through Indirect Immunofluorescence.- High throughput Characterisation of Histidine Phosphorylation sites using UPAX and Tandem Mass Spectrometry.- Proteome Bioinformatics Methods for Studying Histidine Phosphorylation.