Hepatic Stellate Cells

Methods and Protocols

Inbunden, Engelska, 2023

AvRalf Weiskirchen,Scott L. Friedman,Scott L. Friedman

2 959 kr

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This detailed volume explores hepatic stellate cells, which have become a spotlight of liver cell biology due to their pleiotropic functions that extend well beyond extracellular matrix production to pivotal roles in hepatic homeostasis, immunity, and metabolism.

Produktinformation

Utgivningsdatum2023-05-30
Mått178 x 254 x 24 mm
Vikt809 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor309
Upplaga2023
FörlagSpringer-Verlag New York Inc.
ISBN9781071632062

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update.- Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells.- Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells.- Phalloidin Staining for F-Actin in Hepatic Stellate Cells.- Retinyl Ester Analysis by Orbitrap Mass Spectrometry.- Studying Hepatic Stellate Cell Senescence.- Isolation of Hepatic Stellate Cells and Lymphocytes for Co-Culture Systems.- Working with Immortalized Hepatic Stellate Cell Lines.- Induction of Obstructive Cholestasis in Mice.- Mouse Models for Hepatic Stellate Cell Activation and Liver Fibrosis Initiation.- Generation and Culture of Primary Mouse Hepatocyte-HSC Spheroids.- Hepatic Stellate Cell Depletion and Genetic Manipulation.- Human Hepatic Stellate Cells: Isolation and Characterization.- Decellularization of Human Liver to Generate Native Extracellular Matrix for Use in Automated Functional Assays with Stellate Cells.- Multiplex Immunostaining to Spatially Resolve the Cellular Landscape in Human and Mouse Livers.- Single Cell Secretome Analyses of Hepatic Stellate Cells: Aiming for Single Cell Phenomics.- Hepatic Stellate Cells Targeting Using Peptide-Modified Biologicals.- Experimental Workflow for Preclinical Studies of Human Antifibrotic Therapies.