Hemorrhagic Fever Viruses

Methods and Protocols

Häftad, Engelska, 2018

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Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term.

Produktinformation

Utgivningsdatum2018-08-22
Mått178 x 254 x 24 mm
Vikt829 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor425
FörlagHumana Press Inc.
ISBN9781493983582

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Global Spread of Hemorrhagic Fever Viruses: Predicting Pandemics.- An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses.- Preliminary Classification of Novel Hemorrhagic Fever-Causing Viruses Using Sequence-Based Pairwise Sequence Comparison (PASC) Analysis.- Epidemiological Surveillance of Viral Hemorrhagic Fevers with Emphasis on Clinical Virology.- Diagnostics for Lassa Fever: Detecting Host Antibody Responses.- Sampling Design and Mosquito Trapping for Surveillance of Arboviral Activity.- Epidemiological Surveillance of Rodent-Borne Viruses (Roboviruses).- Entry Studies of New World Arenaviruses.- Studies of Lassa Virus Cell Entry.- A Cell-Cell Fusion Assay to Assess Arenavirus Envelope Glycoprotein Membrane-Fusion Activity.- Assays to Assess Arenaviral Glycoprotein Function.- Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus.- Assays to Demonstrate the Roles of Arenaviral Nucleoproteins (NP)in Viral RNA Synthesis and in Suppressing Type I Interferon.- Intracellular Detection of Viral Transcription and Replication using RNA FISH.- Hemorrhagic Fever Virus Budding Studies.- Murine Models for Viral Hemorrhagic Fever.- Testing Experimental Therapies in a Guinea Pig Model for Hemorrhagic Fever.- A Primate Model for Viral Hemorrhagic Fever.- A Primary Human Liver Cell Culture Model for Hemorrhagic Fever Viruses.- Protocol for the Production of a Vaccine Against Argentine Hemorrhagic Fever.- Detection of Virus-Antibody Immune Complexes in Secondary Dengue Virus Infection.- Future Approaches to DNA Vaccination Against Hemorrhagic Fever Viruses.- Identifying Restriction Factors for Hemorrhagic Fever Viruses: Dengue and Junín.- Determining the Virus Life-Cycle Stage Blocked by an Antiviral.- Retrovirus-Based Surrogate Systems for BSL-2 High-Throughput Screening of Antivirals Targeting BSL-3/4 Hemorrhagic Fever-Causing Viruses.- Protocols to Assess Coagulation following In Vitro Infection with Hemorrhagic Fever Viruses.