Hedgehog Signaling

Methods and Protocols

Inbunden, Engelska, 2021

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This detailed book explores the technical breakthroughs with biophysical and cell biological approaches that have advanced the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal, indicating the important role of sterols in this pathway.

Produktinformation

Utgivningsdatum2021-09-27
Mått178 x 254 x 20 mm
Vikt685 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor244
FörlagSpringer-Verlag New York Inc.
ISBN9781071617007

Tillhör följande kategorier

Onkologi inom Medicin
Biokemi inom Naturvetenskap och teknik

Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells.- Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-Coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay.- Analysis of Protein Cholesterylation by Biorthogonal Labeling.- An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched.- Imaging of the Ciliary Cholesterol Underling the Sonic Hedgehog Signal Transduction.- Studying Hedgehog Signaling during Mouse Neural Tube Development.- Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling.- The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells.- Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release.- Expression, Purification, and Structure Determination of Human PTCH1–HH-N Complexes.- Characterization of Smoothened Phosphorylation and Activation.- Cell-Based Assays for Smoothened Ubiquitination and Sumoylation.- Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody.- Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems.- Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors.- Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG.- NanoBRET and NanoBiT/BRET-Based Ligand Binding Assays Permit Quantitative Assessment of Small Molecule Ligand Binding to Smoothened.- Measuring Smoothened (SMO)-Mediated Activation of the Gi Protein.- Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases.- A Method for Orthotopic Transplantation of Lung Cancer in Mice.