This book constitutes the refereed proceedings of the 7th International Conference on HCI in Mobility, Transport, and Automotive Systems, MobiTAS 2025, held as part of the 27th HCI International Conference, HCII 2025, which took place in Gothenburg, Sweden, during June 22–27, 2025.A total of 1430 papers and 355 posters included in the HCII 2025 proceedings was carefully reviewed and selected from 7972 submissions.The MobiTAS 2025 proceedings were organized in the following topical sections- Human-Autonomous Vehicle Interaction and User Experience; User Interfaces and Interaction Methods for Mobility; Trust, Transparency, and Comfort in Automated Driving; Pedestrian Interaction and Road Safety in Automated Mobility.