Handbook of Methods in Aquatic Microbial Ecology

Kemp, Paul F.; Cole, Jonathan J.; Sherr, Barry F.; Sherr, Evelyn B.

• Introduction Section I: Isolation of Living Cells 1. Isolation and Enumeration of Anaerobic and Microaerophiiic Bacteria in Aquatic Habitats 2. Isolation and Cultivation of Hyperthermophilic Bacteria from Marine and Freshwater Habitats 3. Isolation of Psychrophilic Bacteria 4. Isolation and Characterization of Bacteriocytes from a Bivalve-Sulfur Bacterium Symbiosis 5. General Techniques for the Isolation and Culture of Marine Protists from Estuarine, Littoral, Psammolittoral, and Sublittoral Waters 6. Long-Term Culture of Marine Benthic Protists 7. Behavior and Bioenergetics of Anaerobic and Microaerobic Protists 8. Culturing Free-Living Marine Phagotrophic Dinoflagellates 9. Enrichment, Isolation, and Culture of Free-Living Heterotrophic Flagellates 10. Determination of Pressure Effects on Flagellates Isolated from Surface Waters 11. Isolation, Cloning, and Axenic Cultivation of Marine Cilliates 12. Isolation and Laboratory Culture of Marine Oligotrichous Ciliates 13. Extraction of Protists in Aquatic Sediments via Density Gradient Centrifugation Section II. Identification, Enumeration, and Diversity 14. Statistical Analysis of Direct Counts of Microbial Abundance 15. Enumeration and Isolation of Viruses 16. Total Count of Viruses in Aquatic Environments 17. Improved Sample Preparation for Enumeration of Aggregated Aquatic Substrate Bacteria 18. Direct Estimates of Bacterial Numbers in Seawater Samples Without Incurring Cell Loss Due to Sample Storage 19. Total and Specific Bacterial Counts by Simultaneous Staining with DAPI and Fluorochrome-Labeled Antibodies 20. Use of RFLPs for the Comparison of Marine Cyanobacteria 21. Use of High-Resolution Flow Cytometry to Determine the Activity and Distribution of Aquatic Bacteria 22. Phytoplankton Analysis Using Flow Cytometry 23. Enumeration of Phototrophic Picoplankton by Autofluorescence Microscopy 24. Estimating Cell Concentration and Biomass of Autotrophic Plankton Using Microscopy 25. Preservation and Storage of Samp