Handbook of Assay Development in Drug Discovery

Inbunden, Engelska, 2006

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The need to screen targets faster and more efficiently, coupled with advances in parallel and multiplex chemical synthesis, has contributed to the increasing use of multiwell assays for drug discovery. The Handbook of Assay Development in Drug Discovery is a reference that describes the complete armament of tools currently available for performing various assay techniques.Featuring contributions from assay developers in the pharmaceutical and vendor communities, the book presents descriptions of methods, laboratory guidelines and protocols used to perform such methods, specific examples of each assay system, and troubleshooting tools. The handbook describes biochemical assay classes as well as non-class specific assay development for cell-based assays. It covers a wide range of target classes—including kinases, proteases, nuclear receptors, and GPCRs—and describes currently employed methods and assay types, such as radioligand binding assays, image analysis assays, enzyme fragment complementation, and bioluminescent and fluorescent-based assays. Designed as a guide to running an assay from start to finish, the Handbook of Assay Development in Drug Discovery is an ideal bench top companion for discovery researchers, laboratory managers, academics, and other scientists involved in drug discovery screening, lead profiling, therapeutic target evaluation, and assay development and implementation in the pharmaceutical and biotechnology industries. Daniel E. Levy, editor of the Drug Discovery Series, is the founder of DEL BioPharma, a consulting service for drug discovery programs. He also maintains a blog that explores organic chemistry.

Produktinformation

Utgivningsdatum2006-01-20
Mått178 x 254 x 32 mm
Vikt1 020 g
FormatInbunden
SpråkEngelska
SerieDrug Discovery Series
Antal sidor482
FörlagTaylor & Francis Inc
ISBN9781574444711

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Apotek och farmaceuter inom Medicin
Tillverkningsteknik inom Naturvetenskap och teknik

Protein Kinases in Drug Discovery: Rationale, Success, and Challenge. An Introduction to the Protein Tyrosine Phosphatase Gene Family and Screening Assay Development. Time-Resolved Fluorescence and Time-Resolved Fluorescence Resonance Energy Transfer. Developement of High-Thoroughput Screening Assays for Kinase Drug Targets using AlphaScreen Technology. Homogeneous HTS Assays for Serine/Threonine Kinases using b-galactosidase Enzyme Fragment Complementation. IMAP Assays for Assaying Protein Kinases. A Homogeneous, Luminescent, High Throughput, and Versatile Assay for Wide Range of Kinases. Proteases as Drug Targets. Comparison of Homogeneous Bioluminescent and Fluorescent Methods for Protease Assays. SPA Receptor Binding Assays. Radioligand Binding Filtration Assay: Full Automation. Nuclear Receptors as Drug Targets. Nuclear Receptor Scintillation Proximity Assays. Homogeneous Assay Development for Nuclear Receptor using the AlphaScreen Technology. Development of Nuclear Receptor Homogeneous Assay using the Lance Technology. Emerging Role of Cell-based Assays in Discovery. The Preparation of Cells for High Content Screening. The Evolution of cAMP Assays. A Homogeneous Fluorescent Polarization Assay for Inositol 1, 4, 5 Trisphosphate (Ins P3). Scintillation Proximity Assay of Inositol Phosphates. Measuring Calcium Mobilization with Gq-Coupled GPCRs using the Fluorometric Imaging Plate Reader (FLIPR). Development of FLIPR-Based HTS Assay for Gi Coupled GPCRs. Aurora Assays. Membrane Potential Based Assays for Ion Channels and Electrogenic Transporters.