Genotoxicity Assessment

Methods and Protocols

Inbunden, Engelska, 2013

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This Methods in Molecular Biology book offers in vitro and in vivo protocols on mutation assays, cytogenetic techniques, primary DNA damage, alternate assays to animal models and updated ICH guidelines. Includes material lists, troubleshooting tips and more.

Produktinformation

Utgivningsdatum2013-07-30
Mått178 x 254 x 31 mm
Vikt1 042 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor463
FörlagHumana Press Inc.
ISBN9781627035286

Tillhör följande kategorier

Bacterial Mutation Assays.- In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay.- Erythrocyte--Based Pig--a Gene Mutation Assay.- Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat Lymphocytes.- In vivo cII, gpt, and Spi- gene Mutation Assays in Transgenic Mice and Rats.- In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests.- Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse.- Chromosomal Aberration Test in Human Lymphocytes.- In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood.- Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells.- Flow Cytometric Determination of Micronucleus Frequency.- Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus Test.- Comparative Genomic Hybridization (CGH) in Genotoxicology.- The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity.- g--H2AX Detection in Somatic and Germ Cells of Mice.- Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers.- The Comet Assay: Assessment of in vitro and in vivo DNA Damage.- The Comet Assay in Human Biomonitoring.- The Comet assay in Marine Animals.- Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo.- 32P--Postlabeling Analysis of DNA Adducts.- Micronucleus Assay with Tetrad Cells of Tradescantia.- The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in Drosophila.- Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH).