Genome Editing Tools and Gene Drives

Reagan MudziwapasiReagan Mudziwapasi is a biotechnology researcher, lecturer and entrepreneur. He is a co-founder and Operations Director at EnviroBiotech Solutions Africa, a company that develops biotechnology-based solutions. Reagan has authored several peer-reviewed journals, is a patent holder and has made several inventions and conducted several consultancies. He has contributed to various discussions on biotechnology, entrepreneurship and energy inter alia. Reagan promotes awareness on genetic engineering and its safe and responsible use. He helps marginalized communities and upcoming inventors protect their intellectual property. Farai Faustos MashiriFarai Faustos Mashiri has been an Agricultural Science and Biology educator since 2009 at various institutions in Zimbabwe and recently South Africa. He got his BSc in Agriculture and Natural Resources in 1999 from Africa University and Post Graduate Diploma in Education from Lupane State University. After graduating, his early career engagements included technical work for private enterprises in the Agricultural sector, mainly in aquaculture and horticulture. He is currently undertaking postgraduate studies in Animal Breeding and Biotechnology. His research interests are in Tilapia breeding, genome editing and gene drives. His areas of interest also span the fields of Bioinformatics, Genomics, and Data Analysis using R and Python. Irvonnie ShokoIrvonnie Shoko is a founder of Divin Chen Agribusiness Solutions where she offers consultancy and training services in livestock production. She joined Midlands State University in 2019 as a part-time lecturer in Animal Breeding and Physiology. Prior to that, she was a research scientist at SIRDC. During her tenure at SIRDC, she helped the company to set up a robust livestock breeding and production project. She is a graduate of the University of Zimbabwe where she obtained a BSc in Animal Science and an MSc in Biotechnology. She is currently working towards an MSc in Animal Breeding and Biotechnology at Lupane State University. She currently lives in Harare, Zimbabwe with her family. She enjoys empowering communities in agribusiness. When not working, she enjoys nature walks and swimming.Berlinda Ncube Berlinda Ncube is a cofounder of Go Science Club where she helps learners come up with science projects. She joined Zezani High School in 2018 as a chemistry teacher. She is a graduate of the National University of Science and Technology where she obtained a BSc Hons in Applied Biology and Biochemistry and Zimbabwe Open University where she obtained a postgraduate Diploma in Education. She is currently working towards obtaining an MSc in Applied Animal Breeding and Biotechnology at Lupane State University. She spends most of her time empowering the Girl Child and enjoys reading novels and surfing the net. She currently lives in Bulawayo, Zimbabwe with her family. Fortune N. JomaneFortune N. Jomane is an animal scientist who specializes in animal breeding and genetics. He holds a BSc in Agriculture (Animal Science), an MSc in Agriculture (Animal Science) and a Ph.D. in Agriculture specializing in Animal Breeding and Genetics. His passion is enhancing animal production through exploiting genetics. He has published several peer-reviewed papers in animal breeding and genetics. He is a senior lecturer and Chairperson of the Department of Animal Science and Rangeland and Lupane State University. He is the secretary for the beef and leather value chain technical assistance project implemented by the Government of Zimbabwe through the Ministry of Industry and Commerce, and the Ministry of Lands, Agriculture, Fisheries, Water and Rural Resettlement. Dr. Ryman ShokoDr. Ryman Shoko is a Senior Lecturer in the Department of Biological Sciences at the Chinhoyi University of Technology. His areas of expertise include molecular biology, proteomics bioinformatics and computational systems biology.Ringisai ChekeraRingisai Chekera is an animal scientist who specializes in animal breeding. She holds a BSc in Agriculture (Animal Science) and an MSc in Agriculture (Animal Breeding and Biotechnology). She has a great passion for building the resilience of livestock farmers through the use of various breeding strategies. She has contributed to the Resilience Knowledge Hub as a researcher in a project implemented under the ZRBF project to enhance the resilience of smallholder poultry farmers. Dr. Rutendo NyamusambaDr. Rutendo Nyamusamba’s passion lies in development and being a voice for the seemingly voiceless. It is this passion that drives her desire for research. She uses a holistic approach in solving food system challenges. Her research and professional background are strongly rooted in Agronomy and Physiology of field crops focusing on Sustainable Crop Production and Weed Science. She is currently working on the integration of livestock in cropping systems for both commercial and small-scale farmers. Dr. Nyamusamba has a Ph.D. in Agronomy with a minor in Statistics; an MSc. in Plant Science from South Dakota State University, USA and a BSc. in Agriculture with an Agribusiness specialty from Africa University in Mutare, Zimbabwe. She teaches modules in Weed Science, Plant Physiology, Crop Production, Sustainable Agriculture and Statistics. Occasionally she assists with some specific modules in Agricultural Economics such as Econometrics.Jemethious DubeJemethious Dube has been an agricultural scientist. He holds a diploma in Agriculture from Esigodini College, a BSc in Agriculture Management, a BSc Special Honors Degree in Crop Science and a Master’s Degree in Business Administration, all from the Zimbabwe Open University. He also holds a postgraduate diploma in education from Lupane State University. He is studying towards an MSc Crop Science (Plant Breeding) with Lupane State University. His passion for the field saw him attaining. He has vast experience working with financial institutions and non-governmental organizations. He is very passionate about fieldwork.Moira Amanda MubaniMoira Amanda Mubani is a very talented and hardworking young scientist. She holds a BSc Honors Degree in Biotechnology and is currently in her final year completing her Master's Degree in Applied Pharmaceutical Science. She is into computational chemistry, where she developed ways of analyzing a substantial amount of data. She uses molecular modeling and bioinformatics to design novel anti-tuberculosis and anticancer drugs. Moira Amanda also works on Ethno-Pharmaceuticals projects where she is involved in cannabis research. Moira has been working at the National Biotechnology Authority (NBA) since October 2019. She was seconded by NBA to work at the African Institute of Biomedical Science and Technology (AiBST) in 2020. At AiBST gained substantial knowledge and experience in Genomics and Bioinformatics technologies. She also on the National Biobank project. Moira was recently seconded to work on the Biobanking project with Dr. Justen Manasa at the University of Zimbabwe innovation hub. This project is key in the establishment of the Zimbabwe Genomics Center. Moira was among the 10 people selected to participate in a hands-on workshop training in advanced genomics technologies organized by the Biomedical Research and Training Institute. This workshop further equipped her with skills in genome sequencing, bioinformatics, big data analysis software and next-generation sequencing in relation to Covid and HIV research. Moira lives in Harare and when she is not at work Moira devotes her time to attending church services and spending quality time with her family.Mr. Clophas Zibusiso NcubeClophas Z. Ncube is an Animal Science and Agribusiness Practitioner. He has over ten years of continuous work experience. He started his professional career by working as a field officer under a USAID-funded project for five years in Victoria Falls and Gwanda districts. Currently, he is working as the national project coordinator of the Green Jobs project for the Food and Agriculture Organisation (FAO) of the United Nations where his main goal is to promote rural youth employment. Prior to this, he was an Agriculture team leader for five years under a USAID-funded project where he was promoting agricultural livelihoods in Gwanda and Bulilima districts. In 2018, he was awarded the Australian awards scholarship to study Agribusiness concentrating on value chains. Mr. Ncube is a holder of a Master of Science Degree in Agribusiness from Africa University in Mutare, Zimbabwe, a BSc in Agricultural Science and a BSc in Agricultural Science Honors Degree in Animal Science. He is currently a student for the Master of Science Degree in Animal Breeding and Biotechnology at Lupane State University. When he is not working or studying, Mr. Ncube enjoys spending time with his family in Bulawayo, Zimbabwe.Mpumuzi Masuku Mpumuzi Masuku is a final year student at Lupane State University studying towards a Master of Science Degree in Animal Breeding and Biotechnology. He holds a BSc Honors Degree in Agriculture (Animal Science) from the University of Zimbabwe. He is detail-oriented and values self-actualization immensely. He aims to leverage proven knowledge of research in Animal Breeding, Molecular Biotechnology and Genomics. Thandanani MoyoThandanani Moyo obtained a BSc (Hons) in Applied Biology & Biochemistry from NUST, Zimbabwe. Currently, he is studying towards an MSc in Animal Breeding and Biotechnology at Lupane State University. His research interests are molecular breeding and animal health. He is currently working as a lab Scientist at a diagnostics laboratory.Charity ChimboCharity Chimbo is a Biology and Agriculture Science educator since 2010. She holds a BSc Honors Degree in Agriculture (UZ), Post Graduate Diploma in education (ZOU) and is studying towards an MSc in Animal Breeding and Biotechnology with LSU. Her research interests are in genomics and bioinformatics.

• Chapter 01 Genome editingChapter 02 Zinc Finger NucleasesChapter 03 TalensChapter 04 megaTALs/ meganucleases Chapter 05 CRISPRChapter 06 Comparison of ZFNs, TALENs, CRISPR, and megaTALsChapter 07 Gene DrivesChapter 08 Supernumerary B chromosomesChapter 09 Suppression drive Chapter 10 Maternal Effect Dominant Embryonic Arrest (Medea) systemChapter 11 Heritable microorganisms: WolbachiaChapter 12 CRISPR gene drivesChapter 13 The Killer–Rescue system

**Description**This volume describes genome editing tools (molecular methods for the targetedalteration of DNA sequences) and gene drives (mechanisms that propagatespecific genes well in excess of the rate expected by chance). True to itsname, it is a very brief but effective treatment of these important topics.This is a stand-alone paperback book without any supporting website or videobackup. At just 100 pages in length, this book very briefly introduces fourapproaches to genome editing and six approaches to implementing gene drives.The book is divided into 13 chapters that range in length from two to 12 pages.The book is part of the Pocket Guides to Biomedical Science. **Purpose**In the preface, the 13 authors describe their principal motivation for writingthe book as a desire to introduce modern methods of genome editing and genedrives to African scientists and students. Indeed, all of the authors areAfrican and are currently affiliated with universities in Zimbabwe. This bookwill easily fulfill the goal of helping inform African researchers andstudents, but its utility goes well beyond that. This book is broadly useful toany biology student or researcher new to the fields of genome editing or genedrives. **Audience**While the authors are motivated by a desire to smooth the way for the use ofgenomic tools in Africa, their writing is so clear and concise that manyaudiences will find the book useful. Undergraduate students will find theconcise descriptions of genomic and gene drives methods useful as they learnabout these approaches. New graduate student researchers and professionalsexploring genomic tools will likewise find the focused writing helpful ingaining a solid initial understanding of the topics covered. The authors havewritten accurately and effectively about their topics and provide practicalexamples of applications as well as discussions of the pros and cons ofmethods. **Features**All chapters in this book are brief. The opening chapter gives a quickorientation to genome editing. This is followed by five chapters on genomeediting approaches. Descriptions of four genome editing methods, zinc fingernucleases, TALENs, MegaTALs, and CRISPR, are followed by a chapter that offersa convenient comparison of the methods. The remaining six chapters describevarious methods for applying gene drives. Each chapter contains a discussion ofapplications and describes the pros and cons of the method. The book alsocontains a helpful glossary, a long list of references cited, and a good index.**Assessment**This is a highly useful book that, in its brief 100 pages, captures the essenceof four contemporary approaches to genome editing and six ways in which methodsof gene drives can be applied to captive and natural populations. The brevityof this book and its comparative lack of research data set it apart from otherbooks covering the same topics. This book is for those who are new to genomeediting and gene drives. Without getting bogged down in research details,readers with a biology background who are unfamiliar with the methods discussedwill come away with a working understanding of the methods and approachesdescribed. - Mark F. Sanders, PhD (UC Davis College of Biological Sciences)