Edmund C. Lattime is Professor of Surgery at the Robert Wood Johnson Medical School and Deputy Director, The Cancer Institute of New Jersey. Dr. Lattime studies tumor immunology and immunotherapy focusing on the tumor-host interaction and the tumor microenvironment. While faculty at Sloan Kettering and then Thomas Jefferson University, his translational studies led to the development and Phase I testing of a novel Vaccinia-GMCSF construct designed to enhance the development of antitumor immunity via infection/transfection of the tumor microenvironment. Based on his mechanistic studies of immune escape mechanisms, his group recently developed and is testing a poxvirus-based immunization strategy, which uses antigen encoding poxvirus delivered to the tumor microenvironment, in patients with locally-advanced pancreatic cancer. Stanton L Gerson is Director of the Case Comprehensive Cancer Center & the National Center for Regenerative Medicine at Case Western Reserve University and Director of University Hospitals Seidman Cancer Center in Cleveland. Dr. Gerson studies DNA repair, stem cells and cancer therapy. He showed that over-expression of the MGMT DNA repair gene could prevent cancer and that a mutant form of MGMT protects hematopoietic stem cells from chemotherapy using lentiviral gene therapy. He has interrogated MGMT, MMR and BER DNA repair pathways as targets for cancer therapy, and proposed that methoxyamine would block base excision repair used in combination with chemotherapy. Dr. Gerson also directed the initial use of mesenchymal stem cells (MSCs) in bone marrow transplantation & in gene therapy.

• Introduction; Overview of Viral and Non-Viral Vector Technologies; DNA Integration for Monitoring Outcome in Gene Therapy; Multiple Treatment Cycles and Gene Therapy; RNA interference-based therapy for cancer; Targeted systemic delivery of therapeutic siRNA; Lentiviral-vector Common Integration Sites; Selective Replicating Adenovirus and Tumor Necrosis; Cancer Suicide Gene Therapy; Adenovirus-retrovirus Hybrid Vectors ; Electroporation gene therapy; Biomaterials-based technologies for therapeutics and imaging; RNA Inhibition gene therapy; Monocytogenes-based DNA Delivery System; Toxin based Cancer Gene Therapy; Overview: Rationale use of oncolytic virus in clinical applications; Clinical development directions in oncolytic viral therapy; Selectively replicating herpes simplex viral vector; Oncolytic Vaccinia Virus for Cancer Therapy; Lister strain Vaccinia Virus; Modification of Mammalian Reoviruses; Systemic Delivery of Oncolytic adenoviruses ; HIF-activated Oncolytic Adenoviruses; Overview of Immune Targets and Pathways; Overview of Gene Therapy with Gene-modified T Cells; Genetically Engineered (TCR) T cells for adoptive therapy; Viral-based Strategies for Combined Vaccine/microenvironment Modulation; Dendritic Cell Vaccines; Recombinant Poxvirus Vaccines; Provenge® and Genetic Fusion Vaccines; Lentivector Vaccines; In vivo Immune Gene Therapy of Solid Tumours; Chemotherapy Delivered after Viral Immunogene Therapy; Overview: selecting effective targets for gene therapy of cancer; Targeting Telomerase-expressing Cancer Cells; Adenovirus-mediated shRNAs ; Adenovirus-mediated SOCS3 Gene Transfer ; p53-based Cancer Therapy; Strategies for tumor-directed delivery of siRNA; Overview of Drug Resistance and Manipulation; Clinical Trials using LV P140KMGMT for Gliomas; Delivery of Liposome-mediated MGMT-siRNA; Cytidine Deaminase Gene Therapy for Recurrent Cancer; Tissue Specific Gene Therapy; Radiation Therapy; Overview of angiogenesis and target identification; Combination of Notch and Ephrin- targeted Therapy; Effects of sustained VEGF blockade on the tumor microenvironment; Clinical studies of Aflibercept (VEGF Trap); Overview of Molecular Imaging Strategies Using Gene-based Vectors; NIS for Radio Gene Therapy; Genetically Engineered Salmonella; NIS Imaging in Cancer; Targeted systemic gene therapy and molecular imaging; Issues and Solutions in Moving Gene-therapy Approaches in Early Phase Trials; Safety and Innovation in Cancer Gene Therapy; Issues and Solutions in Moving Gene-therapy Approaches to Late-phase Trials; FDA Review Process for Cancer Gene Therapy

"The Third Edition…provides crucial updates on the basic and applied sciences of gene therapy. It offers a comprehensive assessment of the field including the areas of suicide gene therapy, oncogene and suppressor gene targeting, immunotherapy, drug resistance gene therapy, and the genetic modification of stem cells." --Anticancer Research 34, 2014"Gene therapy for cancer encompasses reforming genes gone bad, using gene expression to alter the function of both tumor and microenvironment, increasing the sensitivity of cancer cells to killer drugs, and protecting normal cells from such therapies. The studies here cover viral and non-viral vector methodologies: targets and approaches, oncolytic viruses, immunotherapeutics, drug resistance and metabolism gene therapy, cancer therapy imaging vectors, and issues in trail design review and approval." --Reference & Research Book News, December 2013

Edmund C. Lattime is Professor of Surgery at the Robert Wood Johnson Medical School and Deputy Director, The Cancer Institute of New Jersey. Dr. Lattime studies tumor immunology and immunotherapy focusing on the tumor-host interaction and the tumor microenvironment. While faculty at Sloan Kettering and then Thomas Jefferson University, his translational studies led to the development and Phase I testing of a novel Vaccinia-GMCSF construct designed to enhance the development of antitumor immunity via infection/transfection of the tumor microenvironment. Based on his mechanistic studies of immune escape mechanisms, his group recently developed and is testing a poxvirus-based immunization strategy, which uses antigen encoding poxvirus delivered to the tumor microenvironment, in patients with locally-advanced pancreatic cancer. Stanton L Gerson is Director of the Case Comprehensive Cancer Center & the National Center for Regenerative Medicine at Case Western Reserve University and Director of University Hospitals Seidman Cancer Center in Cleveland. Dr. Gerson studies DNA repair, stem cells and cancer therapy. He showed that over-expression of the MGMT DNA repair gene could prevent cancer and that a mutant form of MGMT protects hematopoietic stem cells from chemotherapy using lentiviral gene therapy. He has interrogated MGMT, MMR and BER DNA repair pathways as targets for cancer therapy, and proposed that methoxyamine would block base excision repair used in combination with chemotherapy. Dr. Gerson also directed the initial use of mesenchymal stem cells (MSCs) in bone marrow transplantation & in gene therapy.

• Introduction; Overview of Viral and Non-Viral Vector Technologies; DNA Integration for Monitoring Outcome in Gene Therapy; Multiple Treatment Cycles and Gene Therapy; RNA interference-based therapy for cancer; Targeted systemic delivery of therapeutic siRNA; Lentiviral-vector Common Integration Sites; Selective Replicating Adenovirus and Tumor Necrosis; Cancer Suicide Gene Therapy; Adenovirus-retrovirus Hybrid Vectors ; Electroporation gene therapy; Biomaterials-based technologies for therapeutics and imaging; RNA Inhibition gene therapy; Monocytogenes-based DNA Delivery System; Toxin based Cancer Gene Therapy; Overview: Rationale use of oncolytic virus in clinical applications; Clinical development directions in oncolytic viral therapy; Selectively replicating herpes simplex viral vector; Oncolytic Vaccinia Virus for Cancer Therapy; Lister strain Vaccinia Virus; Modification of Mammalian Reoviruses; Systemic Delivery of Oncolytic adenoviruses ; HIF-activated Oncolytic Adenoviruses; Overview of Immune Targets and Pathways; Overview of Gene Therapy with Gene-modified T Cells; Genetically Engineered (TCR) T cells for adoptive therapy; Viral-based Strategies for Combined Vaccine/microenvironment Modulation; Dendritic Cell Vaccines; Recombinant Poxvirus Vaccines; Provenge® and Genetic Fusion Vaccines; Lentivector Vaccines; In vivo Immune Gene Therapy of Solid Tumours; Chemotherapy Delivered after Viral Immunogene Therapy; Overview: selecting effective targets for gene therapy of cancer; Targeting Telomerase-expressing Cancer Cells; Adenovirus-mediated shRNAs ; Adenovirus-mediated SOCS3 Gene Transfer ; p53-based Cancer Therapy; Strategies for tumor-directed delivery of siRNA; Overview of Drug Resistance and Manipulation; Clinical Trials using LV P140KMGMT for Gliomas; Delivery of Liposome-mediated MGMT-siRNA; Cytidine Deaminase Gene Therapy for Recurrent Cancer; Tissue Specific Gene Therapy; Radiation Therapy; Overview of angiogenesis and target identification; Combination of Notch and Ephrin- targeted Therapy; Effects of sustained VEGF blockade on the tumor microenvironment; Clinical studies of Aflibercept (VEGF Trap); Overview of Molecular Imaging Strategies Using Gene-based Vectors; NIS for Radio Gene Therapy; Genetically Engineered Salmonella; NIS Imaging in Cancer; Targeted systemic gene therapy and molecular imaging; Issues and Solutions in Moving Gene-therapy Approaches in Early Phase Trials; Safety and Innovation in Cancer Gene Therapy; Issues and Solutions in Moving Gene-therapy Approaches to Late-phase Trials; FDA Review Process for Cancer Gene Therapy

"The Third Edition…provides crucial updates on the basic and applied sciences of gene therapy. It offers a comprehensive assessment of the field including the areas of suicide gene therapy, oncogene and suppressor gene targeting, immunotherapy, drug resistance gene therapy, and the genetic modification of stem cells." --Anticancer Research 34, 2014"Gene therapy for cancer encompasses reforming genes gone bad, using gene expression to alter the function of both tumor and microenvironment, increasing the sensitivity of cancer cells to killer drugs, and protecting normal cells from such therapies. The studies here cover viral and non-viral vector methodologies: targets and approaches, oncolytic viruses, immunotherapeutics, drug resistance and metabolism gene therapy, cancer therapy imaging vectors, and issues in trail design review and approval." --Reference & Research Book News, December 2013

Edmund C. Lattime is Professor of Surgery at the Robert Wood Johnson Medical School and Deputy Director, The Cancer Institute of New Jersey. Dr. Lattime studies tumor immunology and immunotherapy focusing on the tumor-host interaction and the tumor microenvironment. While faculty at Sloan Kettering and then Thomas Jefferson University, his translational studies led to the development and Phase I testing of a novel Vaccinia-GMCSF construct designed to enhance the development of antitumor immunity via infection/transfection of the tumor microenvironment. Based on his mechanistic studies of immune escape mechanisms, his group recently developed and is testing a poxvirus-based immunization strategy, which uses antigen encoding poxvirus delivered to the tumor microenvironment, in patients with locally-advanced pancreatic cancer. Stanton L Gerson is Director of the Case Comprehensive Cancer Center & the National Center for Regenerative Medicine at Case Western Reserve University and Director of University Hospitals Seidman Cancer Center in Cleveland. Dr. Gerson studies DNA repair, stem cells and cancer therapy. He showed that over-expression of the MGMT DNA repair gene could prevent cancer and that a mutant form of MGMT protects hematopoietic stem cells from chemotherapy using lentiviral gene therapy. He has interrogated MGMT, MMR and BER DNA repair pathways as targets for cancer therapy, and proposed that methoxyamine would block base excision repair used in combination with chemotherapy. Dr. Gerson also directed the initial use of mesenchymal stem cells (MSCs) in bone marrow transplantation & in gene therapy.

• Introduction; Overview of Viral and Non-Viral Vector Technologies; DNA Integration for Monitoring Outcome in Gene Therapy; Multiple Treatment Cycles and Gene Therapy; RNA interference-based therapy for cancer; Targeted systemic delivery of therapeutic siRNA; Lentiviral-vector Common Integration Sites; Selective Replicating Adenovirus and Tumor Necrosis; Cancer Suicide Gene Therapy; Adenovirus-retrovirus Hybrid Vectors ; Electroporation gene therapy; Biomaterials-based technologies for therapeutics and imaging; RNA Inhibition gene therapy; Monocytogenes-based DNA Delivery System; Toxin based Cancer Gene Therapy; Overview: Rationale use of oncolytic virus in clinical applications; Clinical development directions in oncolytic viral therapy; Selectively replicating herpes simplex viral vector; Oncolytic Vaccinia Virus for Cancer Therapy; Lister strain Vaccinia Virus; Modification of Mammalian Reoviruses; Systemic Delivery of Oncolytic adenoviruses ; HIF-activated Oncolytic Adenoviruses; Overview of Immune Targets and Pathways; Overview of Gene Therapy with Gene-modified T Cells; Genetically Engineered (TCR) T cells for adoptive therapy; Viral-based Strategies for Combined Vaccine/microenvironment Modulation; Dendritic Cell Vaccines; Recombinant Poxvirus Vaccines; Provenge® and Genetic Fusion Vaccines; Lentivector Vaccines; In vivo Immune Gene Therapy of Solid Tumours; Chemotherapy Delivered after Viral Immunogene Therapy; Overview: selecting effective targets for gene therapy of cancer; Targeting Telomerase-expressing Cancer Cells; Adenovirus-mediated shRNAs ; Adenovirus-mediated SOCS3 Gene Transfer ; p53-based Cancer Therapy; Strategies for tumor-directed delivery of siRNA; Overview of Drug Resistance and Manipulation; Clinical Trials using LV P140KMGMT for Gliomas; Delivery of Liposome-mediated MGMT-siRNA; Cytidine Deaminase Gene Therapy for Recurrent Cancer; Tissue Specific Gene Therapy; Radiation Therapy; Overview of angiogenesis and target identification; Combination of Notch and Ephrin- targeted Therapy; Effects of sustained VEGF blockade on the tumor microenvironment; Clinical studies of Aflibercept (VEGF Trap); Overview of Molecular Imaging Strategies Using Gene-based Vectors; NIS for Radio Gene Therapy; Genetically Engineered Salmonella; NIS Imaging in Cancer; Targeted systemic gene therapy and molecular imaging; Issues and Solutions in Moving Gene-therapy Approaches in Early Phase Trials; Safety and Innovation in Cancer Gene Therapy; Issues and Solutions in Moving Gene-therapy Approaches to Late-phase Trials; FDA Review Process for Cancer Gene Therapy

"The Third Edition…provides crucial updates on the basic and applied sciences of gene therapy. It offers a comprehensive assessment of the field including the areas of suicide gene therapy, oncogene and suppressor gene targeting, immunotherapy, drug resistance gene therapy, and the genetic modification of stem cells." --Anticancer Research 34, 2014"Gene therapy for cancer encompasses reforming genes gone bad, using gene expression to alter the function of both tumor and microenvironment, increasing the sensitivity of cancer cells to killer drugs, and protecting normal cells from such therapies. The studies here cover viral and non-viral vector methodologies: targets and approaches, oncolytic viruses, immunotherapeutics, drug resistance and metabolism gene therapy, cancer therapy imaging vectors, and issues in trail design review and approval." --Reference & Research Book News, December 2013