G-Quadruplex DNA

• G-Quadruplexes: From Guanine Gels to Chemotherapeutics.- Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes.- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential.- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis.- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay.- Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy.- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures.- 2-Aminopurine as a Probe for Quadruplex Loop Structures.- Assessing DNA Structures with 125I Radioprobing.- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques.- Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments.- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC.- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography.- Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes.- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands.

From the reviews:“The book G-Quadruplex DNA … provides an excellent collection of important methods for the study of the DNA G-quadruplex structures. … I definitely recommend this book, which can be considered an outstanding source of information that addresses several problems … in the investigation of G-quadruplex structures. The book is appropriate for everyone working in the area of G-quadruplex research, including biologists, biochemists and chemists since it is a worthy guide for both beginners and experienced scientists interested in studying this increasingly attractive field.” (Aldo Galeone, ChemBioChem, Issue 11, September, 2010)