This book constitutes the proceedings of the 16th International Conference on Fundamental Approaches to Software Engineering, FASE 2013, held as part of the European Joint Conference on Theory and Practice of Software, ETAPS 2013, which took place in Rome, Italy, in March 2013. The 25 papers presented in this volume were carefully reviewed and selected from 112 submissions. They are organized in topical sections named: model-driven engineering; verification and validation; software comprehension; analysis tools; model-driven engineering: applications; model transformations; and testing.