Food Allergens

Methods and Protocols

Häftad, Engelska, 2018

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This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies.

Produktinformation

Utgivningsdatum2018-08-07
Mått178 x 254 x 17 mm
Vikt590 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor299
FörlagHumana Press Inc.
ISBN9781493983407

Tillhör följande kategorier

Onkologi inom Medicin

Overview of the Commonly used Methods for Food Allergens.- Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques.- Recombinant Allergen Production in E. Coli.- Recombinant Allergens Production in Yeast.- 2D-Electrophoresis and Immunoblotting in Food Allergy.- Two Dimensional Electrophoresis and Identification by Mass Spectrometry (MS).- Enzyme-Linked Immunosorbent Assay (ELISA).- Detection of Food Allergens by Taqman Real-Time PCR Methodology.- Detection of Food Allergens by Phage Displayed Produced Antibodies.- Protein Microarray-Based IgE-Immunoassay for Allergy Diagnosis.- Basophil Degranulation Assay.- Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy.- Assessment of IgE Reactivity of β-Casein by Western Blotting after Digestion with Simulated Gastric Fluid.- IgE Epitope Mapping using Peptide Microarray Immunoassay.- T Cell Proliferation Assay: Determination of Immunodominant T Cell Epitopes of Food Allergens.- Tetramer Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens.- T-Cell Epitope Prediction.- An Overview of Bioinformatics Tools and Resources in Allergy.- The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid Loaded Dendritic Cells.- Recent Advances in the Detection of Allergens in Foods.