Fluorescence Imaging and Biological Quantification

Raquel Seruca, MD, PhD, is a Professor at Medical Faculty of Porto, vice-president of Ipatimup and Coordinator of the Cancer research program at i3s, both located in Porto, Portugal. Dr. Seruca is an expert in oncobiology with a specific interest in the role of adhesion molecules in cancer and molecular genetics of gastrointestinal cancer. She has more than 200 publications in international peer-review journals, with an h-index of 52, and several national and international awards by her relevant contributions for science and society.Jasjit S. Suri, PhD, MBA, Fellow AIMBE, is an innovator, visionary scientist, and an internationally known world leader. Dr. Suri was crowned with the Director General’s Gold medal in 1980 and elected as a Fellow of American Institute of Medical and Biological Engineering (AIMBE), awarded by National Academy of Sciences in Washington DC in 2004. Dr. Suri has held executive positions, been the Chairman of the IEEE Denver section, and won over 50 awards during his career.João M. Sanches, PhD, is a Professor at the Bioengineering Department (DBE) of the Instituto Superior Técnico (IST), Univers. of Lisbon, and Member of the board of directors of the Institute for Systems and Robotics (ISR/IST). Professor Sanches has about 150 international publications in Biomedical Engineering in statistical signal and image processing methods for biological and medical diagnosis. He is senior member of the IEEE Engineering in Medicine and Biology Society and Member of the Bio Imaging and Signal Processing Technical Committee (BISP-TC) of the IEEE Signal Processing Society.

• Confocal microscopy in the life sciences; Imaging flow cytometry for quantification of cellular parameters; Live-cell imaging: Seeing is believing; Atomic force microscopy: A tool for imaging, manipulating biomolecules, and quantifying single-molecule interactions in biological systems; Confocal Raman microscopy: Imaging the chemistry; Tracking cancer: In vivo imaging techniques; Quantum dots: Concepts, imaging, and therapeutic applications in cancer; Selecting the appropriate in situ proximity ligation assay protocol; Cell detection and joint shape tracking using local image filters; Studying subcellular signaling events in living microglial cells using fluorescence resonance energy transfer-based nanosensors; Membrane trafficking under the microscope, what new imaging technologies have brought to light; Illuminating the cycle of life; Methods for the visualization of circadian rhythms: From molecules to organisms; Measurement of lysosomal ion homeostasis by fluorescence microscopy; Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations; Cancer cell invadopodia: Visualization and quantification tools.

"Microscopy techniques have brought significant improvements and novel implementations to several fields in the past few years. This compendium offers a fresh review of several techniques (fluorescence confocal, Raman and AFM) and their applications to life sciences; it will be useful to the experienced researcher as well as to the student approaching the use and applications of microscopy techniques." — Beniamino Barbieri, ISS, Champaign, Illinois, USA