Del 2280 - Methods in Molecular Biology

Flavins and Flavoproteins

Methods and Protocols

Inbunden, Engelska, 2021

2 429 kr

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This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells.

Produktinformation

Utgivningsdatum2021-03-23
Mått178 x 254 x 23 mm
Vikt787 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor298
FörlagSpringer-Verlag New York Inc.
ISBN9781071612859

Tillhör följande kategorier

Kemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Selection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence.- Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata.- Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata.- Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System.- Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2.- Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra.- Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions.- Redox Titration of Flavoproteins: An Overview.- Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins.- Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms.- Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State.- Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner.- Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity of the Novel Rid (Enamine/Imine Deaminase) Class of Enzymes.- The In Vitro Production of prFMN for Reconstitution of UbiD Enzymes.- Alcohol Oxidase from the Methylotrophic Yeast Ogataea polymorpha: Isolation, Purification, and Bioanalytical Application.- Flavocytochrome b2 of the Methylotrophic Yeast Ogataea polymorpha:Construction of Overproducers, Purification, and Bioanalytical Application.- Mammalian Flavoproteome Analysis Using Label-Free Quantitative Mass Spectrometry.- Alteration of Flavin Cofactor Homeostasis in Human Neuromuscular Pathologies.