Experimental Glycoscience

Glycochemistry

Häftad, Engelska, 2008

Av Naoyuki Taniguchi, Akemi Suzuki, Yukishige Ito, Hisashi Narimatsu, Toshisuke Kawasaki, Sumihiro Hase

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The aim of this book is to provide experimental protocols covering many aspects of glycobiology, glycotechnology, and chemistry: biochemistry, molecular and cellular biology, genetics, physiology, and medicine.

Produktinformation

Utgivningsdatum2008-08-06
Mått155 x 235 x 15 mm
Vikt411 g
FormatHäftad
SpråkEngelska
Antal sidor252
FörlagSpringer Verlag, Japan
ISBN9784431779230

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Structural Analysis of Sugar Chains.- Chemical Liberation of N-linked Oligosaccharides from Glycoproteins.- Release of N-glycans by Enzymatic Methods.- Release of O-glycans by Chemical Methods.- Release of O-glycans by Enzymatic Methods.- Ceramidase and Related Enzymes.- Labeling of Oligosaccharides.- Chemical Labeling of Sialyloligo/polymer.- Separation of Oligosaccharides by 2D HPLC.- Analysis of Oligosaccharides by Capillary Electrophoresis.- Energy-Resolved Mass Spectrometry (ERMS) of Oligosaccharide.- LC/MS of N-Linked Oligosaccharides.- Convenient Structural Characterization of Intact Glycosphingolipids by MALDI-TOF Mass Spectrometry with Increased Laser Power and Cooling Gas Flow.- Structural Analyses of Glycoconjugates by NMR.- Sugar Chain Analysis by Enzymatic Digestion and 2D Mapping by HPLC.- Analysis of Binding Sites of Sugar Chains by Methylation Analysis.- Glycan Profiling.- Monoclonal Antibody as a Clue to Structural Analysis of Bioactive Functional Glycoconjugates.- Microsequencing of Functional Chondroitin Sulfate Oligosaccharides.- Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry.- Structural Analysis of Phospho-Glycosphingolipids in Lower Animals.- Structural Analysis of Polysialic Acid.- Milk Oligosaccharides: Structural Characterization and Future Aspects.- An XML Description of Carbohydrate Structures.- Construction of a Diagnostic Library for Glycans Using Multistage Tandem Mass Spectrometry (MS n ).- A Method for Large-Scale Analysis for N-linked Glycoproteins by the Glycosylation Site-Specific Stable Isotope-Labeling and LC/MS Shotgun Technology.- Mass Spectrometry of Glycopeptides.- Development of MS Method for the Analysis of Sugar Peptide.- Calculations for Saccharides bythe Use of MS Data.- Molecular Modeling of Oligosugar Structures.- Prediction of Sugar-Binding Sites on Proteins.- Frontal Affinity Chromatography: An Effective Analytical Tool for Protein-Sugar Interaction.- Analyses of Sugar-Protein Interactions by NMR.- Surface Plasmon Resonance and Sugar Chip Analysis for Sugar Chain-Protein Interactions.- Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis.- Equilibrium Dialysis.- Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS).- Development of Neoglycoconjugate Probes and Detection of Lectins.- Chemical Synthesis of Sugar Chains.- Synthesis of Glycolipid and Its Application.- Chemo-Enzymatic Synthesis of Glycoconjugates.- Construction of O-Linked Glycopeptide Library Using Human Glycosyltransferases.- Alteration of N-Linked Oligosaccharides.- Stationary Solid-Phase Reaction (SSPR) for Oligosaccharide Synthesis.- Sugar Chain Synthesis by the Use of Cell Functions.- Glyco-Chemistry Cycle System Based on Glycosidases.- Endoglycosidases (Glycosaminoglycans).- Oligosaccharide Synthesis Based on Combinatorial Chemistry and Labo Automation.- Endoglycosidases (Glycoproteins).- Glycosaminoglycans.- Recent Advances in the Production of Mammalian-Type Sugar Chains in Yeast.- Solid-Phase Synthesis of Glycopeptides.- Efficient Synthesis of Oligosaccharides and Synthesis of Pathogen-Associated Molecular Patterns for Their Biofunctional Studies.- Sugar Polymers (Dendrimers and Pendant-Type Linear Polymers).- Rapid Synthesis of Oligosaccharides: Resin Capture-Release Strategy.