This book constitutes the refereed proceedings of the Third International Conference on Biologically Inspired Music, Sound, Art and Design, Evo MUSART 2014, held in Granada, Spain, in April 2014, co-located with the Evo* 2013 events Euro GP, Evo COP, Evo BIO and Evo Applications.The 11 revised full papers presented were carefully reviewed and selected from 30 submissions. They cover a wide range of topics and application areas.