Essential Zebrafish Methods: Cell and Developmental Biology

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

• Overview of the Zebrafish SystemH. William Detrich IIICell Cycles and Development in the Embryonic ZebrafishDonald A. KanePrimary Fibroblast Cell CultureBarry H. PawProduction of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)John H. PostlethwaitAnalysis of Protein and Gene ExpressionTrevor JowettAnalysis of Zebrafish Development Using Explant Culture AssaysHazel SiveConfocal Microscopic Analysis of Morphogenetic MovementsMark S. CooperCytoskeletal Dynamics of the Zebrafish EmbryoLilianna Solinica-KrezelHaploid Screens and Gamma-Ray MutagenesisCharline WalkerPositional Cloning of Mutated Zebrafish GenesWill TalbotThe Zebrafish GenomeJohn PostlethwaitAnalyzing Axon Guidance in the Zebrafish Retinotectal SystemChi-Bin ChienAnalysis of the Cell Cycle in Zebrafish EmbryosJames F. AmatrudaCellular Dissection of Zebrafish HematopoiesisDavid TraverCulture of Embryonic Stem Cell Lines from ZebrafishPaul CollodiNeurogenesisLaure Bally-CuifTime-Lapse Microscopy of Brain DevelopmentScott E. FraserDevelopment of the Peripheral Sympathetic Nervous System in ZebrafishPaul D. HenionApproaches to Study Neurogenesis in the Zebrafish RetinaJarema MalickiInstrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms David W. TankDevelopment of Cartilage and BoneThomas F. SchillingMorphogenesis of the Jaw: Development Beyond the EmbryoPamela C. YelickCardiac DevelopmentDidier Y. R. StainierZebrafish Kidney DevelopmentIain A. DrummondRetroviral-Mediated Insertional Mutogenesis in ZebrafishAdam Amsterdam Genetic Screens for Maternal-Effect MutationsFrancisco PelegriBehavior Screening Assays in ZebrafishHerwig BaierA High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in ZebrafishCecelia B. MoensTransgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable ElementKoichi KawakamiMolecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in ZebrafishCharles LeeThe Zon Laboratory Guide to Positional Cloning in ZebrafishLeonard I. ZonSleeping Beauty Transposon for Efficient Gene DeliveryStephen C. EckerTransgene Manipulation in Zebrafish by Using RecombinasesGary W. StuartHighly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceIJoachim WittbrodtCloning Zebrafish by Nuclear TransferShuo LinSpatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization ScreeningChristine ThisseGenetic Backgrounds, Standard Lines, and Husbandry of ZebrafishBill TrevarrowCommon Diseases of Laboratory ZebrafishJennifer L. MatthewsZebrafish Sperm CryopreservationThomas Look