Essential Genes and Genomes

Methods and Protocols

Inbunden, Engelska, 2021

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With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods.

Produktinformation

Utgivningsdatum2021-10-29
Mått178 x 254 x 30 mm
Vikt1 034 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor434
FörlagSpringer-Verlag New York Inc.
ISBN9781071617199

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik

A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9.- CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines.- Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes.- Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans.- Identification of Essential Genes Using Sequential CRISPR and siRNA Screens.- A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines.- New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen.- A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria.- A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum.- The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa.- Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing.- Using Genome Scale Mutant Libraries to Identify Essential Genes.- Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing.- The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems.- A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus.- The Application of Transposon Insertion Sequencing in Identifying Essential Genes in B. fragilis.- Repressible Promoter System to Study Essential Genes in Mycobacteria.- A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification ofCell Essential Genes.- Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic Balancers.- A Screening Method to Identify Essential Yeast Genes for Responses Towards Spermine.- An Effective Pre-Processing Method for High-Quality Pan-Genome Analysis of Bacillus subtilis and Escherichia coli.- Analysis of Gene Essentiality from TnSeq Data Using Transit.- Geptop 2.0: Accurately Select Essential Genes from the List of Protein-Coding Genes in Prokaryotic Genomes.