Epstein Barr Virus

• Current Trends and Alternative Scenarios in EBV Research.- Epstein-Barr Virus: Clinical Diagnostics.- Establishment of EBV-Infected Lymphoblastoid Cell Lines.- Generation and Infection of Organotypic Cultures with Epstein-Barr Virus.- Affinity Purification-Mass Spectroscopy Methods for Identifying Epstein-Barr Virus-Host Interactions.- The Use of 3D Telomere FISH for the Characterization of the Nuclear Architecture in EBV-Positive Hodgkin’s Lymphoma.- Analysis of EBV Transcription Using High-Throughput RNA Sequencing.- Analysis of Viral Promoter Usage in EBV-Infected Cell Lines: A Comparison of qPCR following Conventional RNA Isolation and Nuclear Run-On Assay.- Analysis of Viral and Cellular MicroRNAs in EBV Infected Cells.- Isolation and Characterization of Exosomes Released by EBV Immortalized Cells.- Functional Analysis of Exosomes Derived from EBV-Infected Cells.- Terminal Repeat Analysis of EBV Genomes.- Characterization of EBVPromoters and Coding Regions by Sequencing PCR-Amplified DNA Fragments.- The Use of Chromatin Precipitation Coupled to DNA Sequencing (ChIP-Seq) for the Analysis of Zta Binding to the Human and EBV Genome.- Analysis of Viral Epigenotypes Using Bisulfite Sequencing: A Detailed Protocol for the Crucial Bisulfite Modification and PCR Amplification Steps.- Analysis of Viral Epigenotypes using Chromatin Immunoprecipitation.- Mice with Reconstituted Human Immune System Components as a Tool to Study Immune Cell Interactions in EBV Infection.- Generation and Analysis of Humanized Mouse Model of EBV Infection.- EBV-Directed T Cell Therapeutics for EBV-Associated Lymphomas.

“This is an excellent book with a good number of color illustrations providing recent immunological and cytogenetic methods and protocols aimed at finding easy solutions to unresolved problems in the field of Epstein Barr virus. … The book is appropriate for students and scientists interested in Epstein Barr Virus (EBV) and EBV diagnostics. … The book has high quality color illustrations to promote easy understanding of this complex subject.” (Omer Iqbal, Doody's Book Reviews, April, 2017)