Epithelial Cell Culture Protocols

Häftad, Engelska, 2010

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Well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Epithelial Cell Culture Protocols offers a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.

Produktinformation

Utgivningsdatum2010-11-10
Mått152 x 229 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor406
FörlagHumana Press Inc.
ISBN9781617372414

Tillhör följande kategorier

Lungmedicin inom Medicin

Human Lens Epithelial Cell Culture.- Human Airway Epithelial Cell Culture.- Rat Gastric Mucosal Epithelial Cell Culture.- Thymic Epithelial Cell Culture.- Bile Duct Epithelial Cell Culture.- Liver Epithelial Cell Culture.- Pulmonary Epithelial Cell Culture.- Prostate Epithelial Cell Isolation and Culture.- A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier.- The Blood-CSF Barrier in Culture.- An In Vitro Model of Differentiated Human Airway Epithelia.- Primary Mouse Keratinocyte Culture.- Analyzing Apoptosis in Cultured Epithelial Cells.- Keratins as Markers of Epithelial Cells.- Epithelial Cell Integrins.- Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium.- Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces.- Transplantation of Cultured Epithelial Cells.- An Outgrowth Culture System of Normal Human Epithelium.- Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract.- Applications of Epithelial Cell Culture in Studies of Drug Transport.- X-Ray Microanalysis of Epithelial Cells in Culture.- ENU Mutagenesis of Rat Mammary Epithelial Cells.- ENU-Induced Ovarian Cancer.- Measurement of Membrane Capacitance in Epithelial Monolayers.- Assessing Epithelial Cell Confluence by Spectroscopy.- Co-Cultivation of Liver Epithelial Cells with Hepatocytes.- Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium.- Establishing Epithelial-Immune Cell Co-Cultures.- Explant Cultures of Embryonic Epithelium.- Bacterial Interactions with Host Epithelium In Vitro.

"The book could be used effectively by experienced or novice basic science researchers or clinicians since the protocols are written clearly and concisely. There are 31 brief chapters in this book and about 60 authors. They are experienced and knowledgeable researchers in the field...The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to culture these cells...would be quite useful for anyone wishing to place epithelial cells in culture."-Doody's Health Sciences Book Review Journal