Epigenetics Methods

Dr. Tollefsbol is a Distinguished Professor at the University of Alabama at Birmingham. He holds doctorates in molecular biology and osteopathic medicine, trained with National Academy of Science members and has over 200 peer-reviewed publications. Reports highlighting the translatability to the lay public of the discoveries made in his laboratory have appeared in international media representing >50 million readers. Dr. Tollefsbol has been featured as an Investigator in the Spotlight by the NIH as well as a Scientist in the Spotlight by ScienceNow. He is an Associate Editor for Frontiers in Genetics, a contributor to Lewin’s GENES classic textbook and Lead Editor for Elsevier’s Translational Epigenetics Series. Dr. Tollefsbol has been named a “Highly Ranked Scholar” worldwide (ScholarGPS). He has been invited for numerous endowed keynote presentations, his research has been highlighted in eScience News and ScienceDaily and he has published 20 scholarly books on topics related to his research.

• Section 1: Overview1. An Overview of Epigenetics MethodsSection 2: DNA Methylation Methods: Gene- or Locus-specific Methylation Analyses 2. Principles of Bisulfite Conversion of DNA and Methylation-specific PCR (MSP) in Biological Research3. Multiplex Methylation Specific Droplet Digital PCR (MS-ddPCR) for Detection and of Rare Methylated AllelesSection 3: DNA Methylation Methods: Global DNA Methylation and Methylomic Analyses 4. Methods for Detection of DNA Methylation in Transposable Elements5. The Illumina Infinium Methylation Assay for Genome-wide Methylation Analyses6. Methylated DNA Immunoprecipitation Sequencing: Methodological Principles and Applications7. Multiplex Analyses Using Methylation Sensitive Restriction Enzyme coupled qPCR and NGS8. Advances in Whole Genome Methylomic SequencingSection 4: DNA Methylation Methods: Additional Technologies9. Analyzing DNA Methyltransferase Enzymatic Activity: Basic Methods and Applications10. Technologies and Applications for the Assessment of 5-HydroxymethylcytosineSection 5: Histone Modification Methods11. The Chromatin Immunoprecipitation (ChIP) Assay and ChIP-qPCR12. CUT&RUN and CUT&Tag for histone modification and transcription factor binding analyses13. Deep Sequencing for Histone Modifications Employing ChIP-seq Analyses14. ChIP in Series: Sequential ChIP15. The ChIP-exo Method to Study DNA-protein interactions and Chromatin Organization at Near-Nucleotide Resolution16. ATAC-seq Method Applied to Embryonic Germ Cells and Neural Stem Cells from Mouse: Practical 17. Combinatorial ChIP and Bisulfite Sequencing (BisChIP-seq)Section 6: Epigenetic Methods for Evaluating Chromatin Higher Order 18. 3D DNA FISH for Analyses of Nuclear Chromatin ArchitectureSection 7: Enzyme Analyses of Histone Modifications19. Assessment of Histone-Modifying Enzymes Involved in Epigenetic Gene RegulationSection 8: Assessment of Non-Coding RNA as an Epigenetic Modulator20. Detection and Quantification of MicroRNAs (miRNAs) and High Throughput miRNAs Profiling21. RIP-seq in Epigenetic Analyses22. Chromatin Isolation by RNA Purification (ChIRP) and Its ApplicationsSection 9: Additional Epigenetics Methods23. Single-Cell Epigenomics: Methods and Translation24. CRISPR/Cas9 Technologies in Epigenetics Research25. Epigenome-Wide Association Study (EWAS) Methods and Applications26. Systems Biology Methods to Study the Epigenome and Metabolic- epigenetic Interactions27. Forensic Epigenetics Methods and ApplicationsSection 10: Future Directions in Epigenetics Methodology 28. Advances in Epigenetic Techniques to Study Development and Diseases