Enzymes of Epigenetics Part B

Dr. Marmorstein obtained his Ph.D. in Chemistry from the University of Chicago and, following a postdoctoral fellowship at Harvard University and a faculty appointment at The Wistar Institute, he joined the faculty at the Perelman School of Medicine at the University of Pennsylvania. Dr. Marmorstein is currently a Professor in the Department of Biochemistry and Biophysics and an Investigator in the Abramson Family Cancer Research Institute with adjunct appoints in the Department of Chemistry at the University of Pennsylvania and The Wistar Institute. The Marmorstein laboratory uses a broad range of molecular, biochemical and biophysical research tools centered on X-ray crystal structure determination to understand the chemical basis for the epigenetic regulation of gene expression. The laboratory is particularly interested in gene regulatory proteins and their upstream signaling kinases that are aberrantly regulated in cancer and other age-related disorders such as obesity and Alzheimer’s disease, and the use of high-throughput small molecule screening and structure-based design strategies towards the development of protein-specific small-molecule probes of protein function and for development into therapeutic agents.

• Epigenetic Technologies1. Identification and Quantification of Histone PTMs using High-Resolution Mass SpectrometryKelly R. Karch, Simone Sidoli and Benjamin A. Garcia2. Substrate Specificity Profiling of Histone-Modifying Enzymes by Peptide MicroarrayEvan M. Cornett, Bradley M. Dickson, Robert M. Vaughan, Swathi Krishnan, Raymond C. Trievel, Brian D. Strahl, and Scott B. Rothbart3. ArrayNinja: An Open Source Platform for Unified Planning and Analysis of Microarray ExperimentsBradley M. Dickson, Evan M. Cornett, Zachary Ramjan and Scott B. Rothbart4. Chemical Biology Approaches for Characterization of Epigenetic RegulatorsDalia Barsyte-Lovejoy, Magdalena M Szewczyk, Panagiotis Prinos, Evelyne Lima-Fernandes, Suzanne Ackloo and Cheryl H. Arrowsmith5. Mapping Lysine Acetyltransferase-Ligand Interactions by Activity-Based CaptureDavid C. Montgomery and Jordan L. Meier6. Investigating Histone Acetylation Stoichiometry and Turnover RateJing Fan, Josue Baeza and John M. Denu7. Rapid Semisynthesis of Acetylated and Sumoylated Histone AnalogsAbhinav Dhall, Caroline E. Weller and Champak Chatterjee8. An IF-FISH Approach for Co-Visualization of Gene Loci and Nuclear Architecture in Fission YeastKyoung-Dong Kim, Osamu Iwasaki  and Ken-ichi NomaSmall Molecule Epigenetic Regulators9. Biology, Chemistry and Pharmacology of SirtuinsAntonio Bedalov, Sarwat Chowdhury and Julian A. Simon10. Synthesis and Assay of SIRT1 Activating CompoundsHan Dai , James L. Ellis , David A. Sinclair and Basil P. Hubbard11. Synthesis and Assays of Inhibitors of MethyltransferasesXiao-Chuan Cai, Kanishk Kapilashrami and Minkui LuoEpigenetics and Biological Connections12. Exploring the Dynamic Relationship between Cellular Metabolism and Chromatin Structure using SILAC-Mass Spec and ChIP-SequencingPhilipp Mews and Shelley L. Berger13. Current Proteomic Methods to Investigate the Dynamics of Histone Turnover in the Central Nervous SystemLorna A. Farrelly, Brian D. Dill, Henrik Molina, Marc R. Birtwistle and Ian Maze14. ChIP-Sequencing to Map the Epigenome of Senescent Cells using Benzonase EndonucleaseTaranjit Singh Rai and Peter D. Adams15. Exploiting Chromatin Biology to Understand ImmunologyJohn L. Johnson and Golnaz Vahed

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience