Del 2767 - Methods in Molecular Biology

Embryo Models In Vitro

Methods and Protocols

Häftad, Engelska, 2025

AvMagdalena Zernicka-Goetz,Kursad Turksen

2 279 kr

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This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo.

Produktinformation

Utgivningsdatum2025-02-07
Mått178 x 254 x 19 mm
Vikt634 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor323
FörlagSpringer-Verlag New York Inc.
ISBN9781071636886

Tillhör följande kategorier

Biokemisk teknik inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik
Regenerativ medicin inom Medicin
Cellbiologi inom Naturvetenskap och teknik

Generation of Human Blastoids from Naïve Pluripotent Stem Cells.- Human Pre-Gastrulation Embryo Culture in 3D Condition.- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models.- Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology.- Endometrial Assembloids to Model Human Embryo Implantation In Vitro.- Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (µPASE).- Generation of 3D Trophoblast Organoids from Human Naïve Pluripotent Stem Cells.- Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells.- Modeling Human Paraxial Mesoderm Development with Pluripotent Stem Cells.- Generation of Stem Cell-Based Mouse Embryo-Like Structures.- Embryonic Spinal Cord Innervation in Human Trunk Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond.- Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts.- Directed Differentiation of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast.- Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos.- Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome Atlas.- Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL.- ShapeMetrics: A 3D Cell Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis.- Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence.- Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell Genomics.- Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence In Situ Hybridization (FISH) in Mammalian Embryos.