Electroporation Protocols

Microorganism, Mammalian System, and Nanodevice

Häftad, Engelska, 2020

AvShulin Li,Lingqian Chang,Justin Teissie

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This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease.

Produktinformation

Utgivningsdatum2020-08-30
Mått178 x 254 x undefined mm
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor196
Upplaga3
FörlagHumana Press Inc.
ISBN9781493997428

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Medicinsk genetik inom Medicin

Cell Size-specific Transfection by Micropillar Array Electroporation.- Soft Electroporation through 3D Hollow Nanoelectrodes.- Micromachining of Polymeric Microfluidic Micro-/Nano-Electroporation Device.- 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation.- Low-Voltage Flow-Through Electroporation Membrane and Method.- Nanofountain Probe Electroporation for Monoclonal Cell Line Generation.- 3D Nanochannel Electroporation for Macromolecular Nucleotides Delivery.- Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells.- Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-function Studies of Target Gene via Knockout.- Microfluidic Device for Localized Electroporation.- Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation.- Targeted in vivo Electroporation Using Nanoengineered Microelectrodes.- Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice.- Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-mediated Counterselection.- Tissue Nano-Transfection of Surgically Exposed Stroma.- Transformation of a Model Microalga, Chlamydomonas reinhardtii without Cell-wall Removal.- Electroporation Transformation Protocol for Phaeodactylum tricornutum.- Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multi-pulse Electroporation.- Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica.- Electroporation Procedures for Genetic Modification of Green Algae Chlorella spp..- Electrotransformation of Saccharomyces cerevisiae.