Electroporation and Electrofusion in Cell Biology

Häftad, Engelska, 2013

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The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material.

Produktinformation

Utgivningsdatum2013-07-15
Mått155 x 235 x 25 mm
Vikt692 g
FormatHäftad
SpråkEngelska
Antal sidor436
FörlagSpringer-Verlag New York Inc.
ISBN9781489925305

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1 Dielectrophoresis and Rotation of Cells.- 2 Cellular Spin Resonance.- 3 Dielectrophoresis: Behavior of Microorganisms and Effect of Electric Fields on Orientation Phenomena.- 4 The Relaxation Hysteresis of Membrane Electroporation.- 5 Electrical Breakdown of Lipid Bilayer Membranes: Phenomenology and Mechanism.- 6 Stochastic Model of Electric Field-Induced Membrane Pores.- 7 Theory of Electroporation.- 8 Leaks Induced by Electrical Breakdown in the Erythrocyte Membrane.- 9 Electroporation of Cell Membranes: Mechanisms and Applications.- 10 Electrofusion Kinetics: Studies Using Electron Microscopy and Fluorescence Contents Mixing.- 11 Electrofusion of Lipid Bilayers.- 12 Role of Proteases in Electrofusion of Mammalian Cells.- 13 Electrofusion of Mammalian Cells and Giant Unilamellar Vesicles.- 14 Cell Fusion and Cell Poration by Pulsed Radio-Frequency Electric Fields.- 15 The Mechanism of Electroporation and Electrofusion in Erythrocyte Membranes.- 16 Producing Monoclonal Antibodies by Electrofusion.- 17 Generation of Human Hybridomas by Electrofusion.- 18 Gaining Access to the Cytosol: Clues to the Control and Mechanisms of Exocytosis and Signal Transduction Coupling.- 19 Gene Transfer by Electroporation: A Practical Guide.- 20 Electropermeabilization and Electrosensitivity of Different Types of Mammalian Cells.- 21 Molecular Genetic Applications of Electroporation.- 22 Plant Gene Transfer Using Electrofusion and Electroporation.- 23 Electric Field-Induced Fusion and Cell Reconstitution with Preselected Single Protoplasts and Subprotoplasts of Higher Plants.- 24 Critical Evaluation of Electromediated Gene Transfer and Transient Expression in Plant Cells.- 25 Transformation Studies in Maize and Other Cereals.- 26 Cells in Electric Fields: Physical and Practical Electronic Aspects of Electro Cell Fusion and Electroporation.- 27 External Electric Field-Induced Transmembrane Potentials in Biological Systems: Features, Effects, and Optical Monitoring.