Downstream Processing of Proteins

Methods and Protocols

Häftad, Engelska, 2010

1 369 kr

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Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology.

Produktinformation

Utgivningsdatum2010-11-19
Mått152 x 229 x 14 mm
Vikt358 g
FormatHäftad
SpråkEngelska
SerieMethods in Biotechnology
Antal sidor229
FörlagHumana Press Inc.
ISBN9781617370755

Tillhör följande kategorier

Biokemisk teknik inom Naturvetenskap och teknik

Downstream Processing in the Biotechnology Industry.- 2 Microbial Cell Disruption by High-Pressure Homogenization.- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration.- 4 High-Performance Tangential Flow Filtration for Protein Separations.- Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation.- Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules.- Application of Chromatography in the Downstream Processing of Biomolecules.- Affinity Ligand Technology.- Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules.- Expanded Bed Adsorption in the Purification of Biomolecules.- Application of HPLC in the Purification of Biomolecules.- Countercurrent Chromatography of Proteins with Polymer Phase Systems.- Practical Aspects and Applications of Radial Flow Chromatography.- Sterile Filtration and Virus Filtration.- Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products.- Validation of Sterilizing Filters in the Biotechnology Industry.

"The contributors are almost exclusively directly and actively involved in practical process applications on a day-to-day basis, which adds considerable credibility to the overall presentation. ... the editor succeeds in his aim, producing a straightforward and uncomplicated directory of the integrated steps necessary to bring a product to market." - Chromatographia "This valuable book is the result of collective work of mostly corporate authors with important experience in downstream processing of proteins in industrial and biotechnological environments...It may be good textbook in industrial biotechnology courses especially because of ample protocols given in this book...The simplicity and clarity of the chapters is contains could be also of a great advantage for food science, which would certainly need to have good bench book...I would recommend highly this unpretentious but highly valuable book also to the readers active in our field."-Nahrung/Food "a worthwhile addition to the reference collections of special and academic libraries that serve scientists interested in the study and isolation of medical products from natural materials."-E-streams Electronic Book Reviews