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Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. This book covers all the main expression systems in current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.
1. Screening expression libraries with antibody and protein probes ; 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies ; 3. Purification of over-produced proteins from E.coli cells ; 4. Production of monoclonal antibodies against proteins expressed in E.coli ; 5. Expression of cloned genes in yeast ; 6. Intraction trap cloning with yeast ; 7. The baculovirus expression system
Robert Eisenthal, Michael J. Danson, Michael Danson, UK) Eisenthal, Robert (Department of Biology and Biochemistry, University of Bath, UK) Danson, Michael (Department of Biology and Biochemistry, University of Bath
M. F. Chaplin, J. F. Kennedy, London) Chaplin, M. F. (Doctor, School of Applied Science, Doctor, School of Applied Science, South Bank University, University of Birmingham) Kennedy, J. F. (Doctor, School of Chemistry, Doctor, School of Chemistry