Del 149 - Practical Approach Series

DNA Cloning 2: A Practical Approach

Expression Systems

Häftad, Engelska, 1995

AvHames Glover,D. M. Glover,Glover,Hames,David M. Glover

1 919 kr

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Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. This book covers all the main expression systems in current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications.

Produktinformation

Utgivningsdatum1995-05-11
Mått157 x 235 x 15 mm
Vikt465 g
FormatHäftad
SpråkEngelska
SerieDel 149 i Practical Approach Series
Antal sidor274
Upplaga2
FörlagIRL Press
ISBN9780199634781

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Medicinsk utrustning och medicinska tekniker inom Medicin
Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik

1. Screening expression libraries with antibody and protein probes ; 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies ; 3. Purification of over-produced proteins from E.coli cells ; 4. Production of monoclonal antibodies against proteins expressed in E.coli ; 5. Expression of cloned genes in yeast ; 6. Intraction trap cloning with yeast ; 7. The baculovirus expression system