DNA and RNA Isolation Techniques for Non-Experts

Inbunden, Engelska, 2022

AvAkash Gautam

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This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work.

Produktinformation

Utgivningsdatum2022-03-30
Mått155 x 235 x 18 mm
Vikt500 g
FormatInbunden
SpråkEngelska
SerieTechniques in Life Science and Biomedicine for the Non-Expert
Antal sidor203
FörlagSpringer Nature Switzerland AG
ISBN9783030942298

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik
Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

1-Basic Idea About Nucleic Acids For Non-Experts.- 2-General Steps During Isolation Of DNA And RNA.- 3-Phenol-Chloroform DNA Isolation Method.- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method.- 5-Spin Column-Based Isolation Of Nucleic Acid.- 6-Isolation Of Plasmid DNA By Alkaline Lysis.- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment.- 8-Lithium Chloride Based Isolation Of DNA And RNA.- 9-Isolation Of Bacteriophage DNA By Peg Method.- 10-DNA Isolation By Chelex Method.- 11-DNA Isolation By Lysozyme And Proteinase K.- 12-Salting-Out Method For Blood Samples.- 13-CTAB Or SDA Based Isolation Of Plant’s DNA.- 14-DNA Extraction By Spooling Method.- 15-Magnetic Bead-Based Nucleic Acid Isolation.- 16-Density Gradient-Based Nucleic Acid Isolation.- 17-DNA Extraction From Agarose Gel Though Paper-Strips.- 18-Transformation Or Genetic Modification Of Cells/Organisms.- 19-Gene Cloning And Vectors.- 20-Polymerase Chain Reaction (PCR).- 21-Southern And Northern Blotting.- 22-Genome Mapping.- 23-Applications Of DNA Sequencing Technologies For Current Research.