This two-volume set LNCS 12645-12646 constitutes the refereed proceedings of the 16th International Conference on Diversity, Divergence, Dialogue, iConference 2021, held in Beijing, China, in March 2021.The 32 full papers and the 59 short papers presented in this two-volume set were carefully reviewed and selected from 225 submissions. They cover topics such as: AI and machine learning; data science; human-computer interaction; social media; digital humanities; education and information literacy; information behavior; information governance and ethics; archives and records; research methods; and institutional management.