This book constitutes the refereed proceedings of the 16th International Baltic Conference on Digital Business and Intelligent Systems, Baltic DB&IS 2024, held in Vilnius, Lithuania, during June 30–July 3, 2024.The 13 full papers and 3 short papers presented here, were carefully reviewed and selected from 36 submissions, and focus on the various fields of Business digitalization and development of intelligent business systems. Abstracts from the keynote speeches from the conference are also included in these proceedings.