Developmental Biology of the Sea Urchin and Other Marine Invertebrates

• 1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae).- Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata.- The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae.- Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis.- Staining and Tracking Methods for Studying Sponge Cell Dynamics.- Microscopy Studies of Placozoans.- Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata.- Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging.- Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization.- Quantifying Cell Proliferation During Regeneration of Aquatic Worms.- In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis.- Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond.- Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation.- Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.- RNA Interference on Regenerating Holothurian Gut Tissues.- ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos.- Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase.- Functional Genomic Studies of Trichoplax adhaerens Neuronal Activity.- A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna.

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