R. Ian Freshney gained a B.Sc. (1960) and Ph.D. (1964) from Glasgow University, where he is on the faculty of Medical Oncology as a senior lecturer and an Honorary Senior Research Fellow. Dr. Freshney currently teaches a number of international basic and specialized cell culture courses, and his research interests include human tumor culture. He is the author of Culture of Animal Cells, a Manual of Basic Technique, and has edited numerous other books on specialized cell culture. Roswitha Pfragner, Ph.D., studied biology at the University of Vienna, Austria and is currently a senior lecturer at the Institute of Pathophysiology. Her research interests include human neuroendocrine tumors, multiple endocrine neoplasia, drug resistance, apoptosis, and genetic events in tumorigenesis.

• Preface viiContributors ixList of Abbreviations xiii1. Growth of Human Lung Tumor Cells in Culture 1Reen Wu2. Culture of Normal and Malignant Gastric Epithelium 23Jae-Gahb Park, Ja-Lok Ku, Hee-Sung Kim, So-Yeon Park and MichaelJ.Rutten3. Establishment of Cell Lines from Colon Carcinoma 67Robert H. Whitehead4. Pancreatic Cancer-Derived Cultured Cells: Genetic Alterations and Application t o an Experimental Model of Pancreatic Cancer Metastasis 81Haruo Iguchi, Kazuhiro Mizumoto, Masaki Shono, Akira Kono and Soichi Takiguchi5. The Establishment and Characterization of Bladder Cancer Cultures In Vitro 97Vivian X. Fu, Steven R. Schwarze, Catherine A. Reznikoff and David F. Jarrard6. Long-term Culture of Normal and Malignant Human Prostate Epithelial Cells 125Robert K. Bright and Jennifer D. Lewis7. The Development of Human Ovarian Epithelial Tumor Cell Lines from Solid Tumors and Ascites 145Anne P.Wilson8. Culture of Cervical Carcinoma Tumor Cell Lines 179Peter Stern, Catherine West and Deborah Burt9. Primary Culture of Human Mammary Tumor Cells 205Valerie Speirs10. Myoepithelium: Methods of Culture and Study 221Sanford H. Barsky and Mary L. Alpaugh11. Multistage Head and Neck Squamous Cell Carcinoma 261Kirsten G. Edington, lsabella J. Berry, Margaret O'Prey, Julie E. Burns, Louise J. Clark, Roy Mitchell, GerryRobertson, David Soutar, Lesley W. Coggins and E. Kenneth Parkinson12. Culture of Melanocytes from Normal, Benign, and Malignant Lesions 289Ruth Halaban13. Establishment and Culture of Human Leukemia-Lymphoma Cell Lines 319Hans G. Drexler14. In Vitro Culture of Malignant Brain Tumors 349John L. Darling15. Culture of Human Neuroendocrine Tumor Cells 373Roswitha Pfragner, Annemarie Behmel, Elisabeth lngolic and Gerhard H. WirnsbergerSuppliers 405Index 419

"This book should be used as reference source for culturing different tumor types. It is a cookbook of sorts, but instead of recipes it has protocols." (E-STREAMS, August 2004)

R. Ian Freshney gained a B.Sc. (1960) and Ph.D. (1964) from Glasgow University, where he is on the faculty of Medical Oncology as a senior lecturer and an Honorary Senior Research Fellow. Dr. Freshney currently teaches a number of international basic and specialized cell culture courses, and his research interests include human tumor culture. He is the author of Culture of Animal Cells, a Manual of Basic Technique, and has edited numerous other books on specialized cell culture. Roswitha Pfragner, Ph.D., studied biology at the University of Vienna, Austria and is currently a senior lecturer at the Institute of Pathophysiology. Her research interests include human neuroendocrine tumors, multiple endocrine neoplasia, drug resistance, apoptosis, and genetic events in tumorigenesis.

• Preface viiContributors ixList of Abbreviations xiii1. Growth of Human Lung Tumor Cells in Culture 1Reen Wu2. Culture of Normal and Malignant Gastric Epithelium 23Jae-Gahb Park, Ja-Lok Ku, Hee-Sung Kim, So-Yeon Park and MichaelJ.Rutten3. Establishment of Cell Lines from Colon Carcinoma 67Robert H. Whitehead4. Pancreatic Cancer-Derived Cultured Cells: Genetic Alterations and Application t o an Experimental Model of Pancreatic Cancer Metastasis 81Haruo Iguchi, Kazuhiro Mizumoto, Masaki Shono, Akira Kono and Soichi Takiguchi5. The Establishment and Characterization of Bladder Cancer Cultures In Vitro 97Vivian X. Fu, Steven R. Schwarze, Catherine A. Reznikoff and David F. Jarrard6. Long-term Culture of Normal and Malignant Human Prostate Epithelial Cells 125Robert K. Bright and Jennifer D. Lewis7. The Development of Human Ovarian Epithelial Tumor Cell Lines from Solid Tumors and Ascites 145Anne P.Wilson8. Culture of Cervical Carcinoma Tumor Cell Lines 179Peter Stern, Catherine West and Deborah Burt9. Primary Culture of Human Mammary Tumor Cells 205Valerie Speirs10. Myoepithelium: Methods of Culture and Study 221Sanford H. Barsky and Mary L. Alpaugh11. Multistage Head and Neck Squamous Cell Carcinoma 261Kirsten G. Edington, lsabella J. Berry, Margaret O'Prey, Julie E. Burns, Louise J. Clark, Roy Mitchell, GerryRobertson, David Soutar, Lesley W. Coggins and E. Kenneth Parkinson12. Culture of Melanocytes from Normal, Benign, and Malignant Lesions 289Ruth Halaban13. Establishment and Culture of Human Leukemia-Lymphoma Cell Lines 319Hans G. Drexler14. In Vitro Culture of Malignant Brain Tumors 349John L. Darling15. Culture of Human Neuroendocrine Tumor Cells 373Roswitha Pfragner, Annemarie Behmel, Elisabeth lngolic and Gerhard H. WirnsbergerSuppliers 405Index 419

"This book should be used as reference source for culturing different tumor types. It is a cookbook of sorts, but instead of recipes it has protocols." (E-STREAMS, August 2004)

R. Ian Freshney gained a B.Sc. (1960) and Ph.D. (1964) from Glasgow University, where he is on the faculty of Medical Oncology as a senior lecturer and an Honorary Senior Research Fellow. Dr. Freshney currently teaches a number of international basic and specialized cell culture courses, and his research interests include human tumor culture. He is the author of Culture of Animal Cells, a Manual of Basic Technique, and has edited numerous other books on specialized cell culture. Roswitha Pfragner, Ph.D., studied biology at the University of Vienna, Austria and is currently a senior lecturer at the Institute of Pathophysiology. Her research interests include human neuroendocrine tumors, multiple endocrine neoplasia, drug resistance, apoptosis, and genetic events in tumorigenesis.

• Preface viiContributors ixList of Abbreviations xiii1. Growth of Human Lung Tumor Cells in Culture 1Reen Wu2. Culture of Normal and Malignant Gastric Epithelium 23Jae-Gahb Park, Ja-Lok Ku, Hee-Sung Kim, So-Yeon Park and MichaelJ.Rutten3. Establishment of Cell Lines from Colon Carcinoma 67Robert H. Whitehead4. Pancreatic Cancer-Derived Cultured Cells: Genetic Alterations and Application t o an Experimental Model of Pancreatic Cancer Metastasis 81Haruo Iguchi, Kazuhiro Mizumoto, Masaki Shono, Akira Kono and Soichi Takiguchi5. The Establishment and Characterization of Bladder Cancer Cultures In Vitro 97Vivian X. Fu, Steven R. Schwarze, Catherine A. Reznikoff and David F. Jarrard6. Long-term Culture of Normal and Malignant Human Prostate Epithelial Cells 125Robert K. Bright and Jennifer D. Lewis7. The Development of Human Ovarian Epithelial Tumor Cell Lines from Solid Tumors and Ascites 145Anne P.Wilson8. Culture of Cervical Carcinoma Tumor Cell Lines 179Peter Stern, Catherine West and Deborah Burt9. Primary Culture of Human Mammary Tumor Cells 205Valerie Speirs10. Myoepithelium: Methods of Culture and Study 221Sanford H. Barsky and Mary L. Alpaugh11. Multistage Head and Neck Squamous Cell Carcinoma 261Kirsten G. Edington, lsabella J. Berry, Margaret O'Prey, Julie E. Burns, Louise J. Clark, Roy Mitchell, GerryRobertson, David Soutar, Lesley W. Coggins and E. Kenneth Parkinson12. Culture of Melanocytes from Normal, Benign, and Malignant Lesions 289Ruth Halaban13. Establishment and Culture of Human Leukemia-Lymphoma Cell Lines 319Hans G. Drexler14. In Vitro Culture of Malignant Brain Tumors 349John L. Darling15. Culture of Human Neuroendocrine Tumor Cells 373Roswitha Pfragner, Annemarie Behmel, Elisabeth lngolic and Gerhard H. WirnsbergerSuppliers 405Index 419

"This book should be used as reference source for culturing different tumor types. It is a cookbook of sorts, but instead of recipes it has protocols." (E-STREAMS, August 2004)