Culture of Hematopoietic Cells

R. IAN FRESHNEY, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

• Partial table of contents: PRIMITIVE PROGENITOR ASSAYS. Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher). Assay for Murine Blast Cell Colonies (N. Katayama & M.Ogawa). Serum-Deprived Cultures of Primary Hematopoietic Cells (G.Migliaccio & A. Migliaccio). COLONY-FORMING ASSAYS FOR MALIGNANT CELLS. Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, etal.). LONG-TERM ASSAYS AND IMMORTALIZATION. Long-Term Culture of Human Myeloid Cells (H. Sutherland & C.Eaves). Immortalization of Hematopoietic Cells (C. MacDonald). PURIFICATION OF PROGENITOR CELLS. Purification of Murine Granulocyte-Macrophage Progenitor Cells(CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper &H. Broxmeyer). OTHER SYSTEMS AND ASSAYS. Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins& R. Fleischman). List of Suppliers. Index.