CRISPR

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels.CRISPR: Methods and Protocolsguides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted fromstandard protocols of DNA, RNA and protein biology.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,CRISPR: Methods and Protocolsprovides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.