Coronaviruses

Methods and Protocols

Inbunden, Engelska, 2020

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This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology.

Produktinformation

Utgivningsdatum2020-08-25
Mått178 x 254 x 24 mm
Vikt728 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor278
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071608999

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik

Coronaviruses: An Updated Overview of Their Replication and Pathogenesis.- Discovery of Novel Coronaviruses in Rodents.- Detection and Discovery of Coronaviruses in Wild Bird Populations.- Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV).- Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing.- Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study.- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation.- The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation.- Isolation and Propagation of Coronaviruses in Embryonated Eggs.- Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures.- Quantification of Coronaviruses by Titration In Vitro and Ex Vivo.- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses.- In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline.- Proximity Labeling for Identification of Coronavirus-Host Protein Interactions.- Using Yeast to Identify Coronavirus-Host Protein Interactions.- Determining How Coronaviruses Overcome the Interferon and Innate Immune Response.- Ribopuromycylation in Coronavirus-Infected Cells.- Visualizing Coronavirus Entry into Cells.- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.