This book constitutes the refereed proceedings of the 13th International Conference on Computer Vision Systems, ICVS 2021, held in September 2021. Due to COVID-19 pandemic the conference was held virtually.The 20 papers presented were carefully reviewed and selected from 29 submissions. cover a broad spectrum of issues falling under the wider scope of computer vision in real-world applications, including among others, vision systems for robotics, autonomous vehicles, agriculture and medicine. In this volume, the papers are organized into the sections: attention systems; classification and detection; semantic interpretation; video and motion analysis; computer vision systems in agriculture.