Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying colorectal tumors. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.

Produktinformation

Utgivningsdatum2000-10-13
Mått155 x 235 x 23 mm
Vikt607 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Medicine
Antal sidor294
Upplaga2001
FörlagHumana Press Inc.
ISBN9780896037670

Tillhör följande kategorier

Onkologi inom Medicin

Microdissection of Histologic Sections.- Isolation of a Purified Epithelial Cell Population from Human Colon.- Xenografting Human Colon Cancers.- Comparative Genomic Hybridization Technique.- Fluorescence In Situ Hybridization.- Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer.- The SURF Technique.- Microsatellite Instability Testing.- Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer.- Mutation Detection in Colorectal Cancers.- Mutation Detection inTGF-? Receptors.- Direct Analysis for Familial Adenomatous Polyposis Mutations.- I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer.- Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas.- Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors.- Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations.- 17 Direct Sequencing for Peutz-Jeghers Gene LKB1 (STK11) Mutations.- 18 Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations.- Genetic Analysis Using Microarrays.- Cclooxygenase-2 (COX-2) Protein Expression by Western Blotting.- The Immunohistochemical Method.- Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids.- Adhesion Signaling Through Integrins.- Electrophoretic Mobility Shift Assay (EMSA).- Transfection Assays for Transformation of Model Colonic Cell Lines.- Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay.- Gene Transfer into the Colonic Mucosa.

"This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, "The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench." A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques...The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research." - Cancer Forum "...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer." - Comprehensive Therapy