Del 0 - Methods in Molecular Biology

Citrus Tristeza Virus

Methods and Protocols

Häftad, Engelska, 2020

AvAntonino F. Catara,Moshe Bar-Joseph,Grazia Licciardello

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This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae.

Produktinformation

Utgivningsdatum2020-08-14
Mått178 x 254 x 15 mm
Vikt496 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor247
FörlagHumana Press Inc.
ISBN9781493995608

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A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies.- A Brief Historical Account of the Family Closteroviridae.- Phenotyping Biological Properties of CTV Isolates.- CTV Vectors and Interactions with the Virus and Host Plants.- Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues.- Detection of Citrus Tristeza Virus and Co-Infecting Viroids.- Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP.- Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus.- Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device.- Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Ampliﬁcation (RT-LAMP) Assay.- Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome.- Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus.- Analysis of Genotype Composition of Citrus Tristeza Virus Populations Using Illumina Miseq Technology.- Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction.- Proteomic Response of Host Plants to Citrus Tristeza Virus.- Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment.- Methods for Producing Transgenic Plants Resistant to CTV.