Cholesterol Homeostasis

Methods and Protocols

Häftad, Engelska, 2018

1 309 kr

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This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell.

Produktinformation

Utgivningsdatum2018-07-20
Mått178 x 254 x 27 mm
Vikt592 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor300
FörlagHumana Press Inc.
ISBN9781493983254

Tillhör följande kategorier

Cellbiologi inom Naturvetenskap och teknik

An Overview of Cholesterol Homeostasis.- Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol Binding Domains in Membrane Proteins.- Structural Stringency of Cholesterol for Membrane Protein Function utilizing Stereoisomers as Novel Tools: A Review .- Manipulating Cholesterol Status within Cells.- Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells.- The use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolaemia and Atherosclerosis.- CRISPR/Cas9-mediated Generation of Niemann-Pick C1 Knockout Cell Line.- Quantitative Measurement of Cholesterol in Cell Population Using Flow Cytometry and Fluorescent Perfringolysin O*.- Transport Assays for Sterol-binding Proteins: Stopped-flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein.- Synthesis and Live-cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport.- Measurement of Cholesterol Transfer fromLysosome to Peroxisomes Using an In Vitro Reconstitution Assay.- Measurement of Mitochondrial Cholesterol Import using a Mitochondria-targeted CYP11A1 Fusion Construct.- Identifying Sterol Response Elements within Promoters of Genes.- Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion.- Determining the Topology of Membrane-bound Proteins using PEGylation.- Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry.- Sterol Analysis by Quantitative Mass Spectrometry.- Measurement of Rates of Cholesterol and Fatty Acid Synthesis in vivo using Tritiated Water.- Methods for Monitoring ABCA1-dependent Sterol Release.- ABC-transporter Mediated Sterol Export from Cells using Radio-Labeled Sterols.- Measurement of Macrophage-specific In Vivo Reverse Cholesterol Transport in Mice.