Chemotaxis

• Microfluidic Techniques for the Analysis of Bacterial Chemotaxis.- Prokaryotic Phototaxis.- Photoorientation in Photosynthetic Flagellates.- Dictyostelium Slug Phototaxis.- Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signal for Cell Migration.- Chemotropism During Yeast Mating.- Group Migration and Signal Relay in Dictyostelium.- Quantitative Analysis of Distal Tip Cell Migration in C. elegans.- Inflammation and Wound Healing in Drosophila.- Neutrophil Motility In Vivo Using Zebrafish.- Chemotaxis in Neutrophil-Like HL-60 Cells.- Chemokine Receptor Dimerization and Chemotaxis.- Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes.- Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy.- In Vivo Assay for Tumor Cell Invasion.- Quantitative Studies of Neuronal Chemotaxis in 3D.- Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylation in Dictyostelium.- Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase Activation.- Quantifying In Vivo Phosphoinositide Turnover in Chemotactically Competent Dictyostelium Cells.- In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum.- Chemokine Receptor Signaling and HIV Infection.- Spatiotemporal Stimulation of Single Cells Using Flow Photolysis.- Spatiotemporal Regulation of Ras-GTPases During Chemotaxis.- FRAP Analysis of Chemosensory Components of Dictyostelium.- Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging, and Single-Molecule Imaging.- Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis.- Analysis of Actin Assembly by In Vitro TIRF Microscopy.- Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells.- Imaging B-Cell Receptor Signaling by Single-Molecule Techniques.- Light Microscopy to Image and Quantify Cell Movement.- Mathematics of Experimentally Generated Chemoattractant Gradients.- Modeling Spatial and Temporal Dynamics of Chemotactic Networks.- Computational Modeling of Signaling Networks for Eukaryotic Chemosensing.

From the reviews: "The purpose is to provide an overview of and practical guidance on the diverse methodologies involved in chemotaxis research. ... Each chapter includes an introduction to the topic, lists of necessary materials and reagents, laboratory protocol, and notes on troubleshooting and avoiding pitfalls, followed by current references. ... This will serve as a valuable resource for students and scientists seeking practical guidance on diverse methodologies in chemotaxis research." (Omer Iqbal, Doody's Review Service, April, 2010)